Liu Wanli, Tao Jiuchen, Wang Heng, Zhao Wenge, Liu Peng
College of Life Science and Technology, Harbin Normal University, Harbin, Heilongjiang, P.R. China.
Mitochondrial DNA B Resour. 2017 Jul 7;2(2):383-384. doi: 10.1080/23802359.2017.1347841.
In this study, the complete mitogenome sequence of (Anura: Ranidae) is first determined using long PCR. It is a circular molecule of 17,733 bp in length (GenBank accession no. KU641020). Similar to the typical mtDNA of amphibians, the complete mtDNA sequence of contained 2 rRNA genes (12S rRNA and 16S rRNA), 22 tRNA genes, 13 protein-coding genes (PCGs), and a control region (D-loop). The nucleotide composition was 28.1% A, 26.8% C, 14.8% G, and 30.3% T. Mitochondrial genome analyses based on the NJ method yield phylogenetic trees, including the 13 already reported Ranidae family frogs. The molecular data presented here provide a useful tool for systematic analyses of genus and .
在本研究中，首次使用长PCR技术确定了（无尾目：蛙科）的完整线粒体基因组序列。它是一个长度为17733 bp的环状分子（GenBank登录号：KU641020）。与两栖动物典型的线粒体DNA相似，该物种的完整线粒体DNA序列包含2个rRNA基因（12S rRNA和16S rRNA）、22个tRNA基因、13个蛋白质编码基因（PCGs）和一个控制区（D-loop）。核苷酸组成分别为：A占2
