Liu Peng, Wang Heng, Zhao Wenge
College of Life Science and Technology, Harbin Normal University, Harbin, Heilongjiang, P.R. China.
Mitochondrial DNA B Resour. 2017 Jul 25;2(2):424-425. doi: 10.1080/23802359.2017.1357444.
In this study, the complete mitogenome sequence of (Anura: Ranidae) is determined using long PCR. It is a circular molecule of 20,571 bp in length (GenBank accession no. MF370348). The complete mtDNA sequence of contained 2 rRNA genes (12S rRNA and 16S rRNA), 22 tRNA genes, 13 protein-coding genes (PCGs) and 2 control regions (D-loops). The nucleotide composition was 28.6% A, 26.8% C, 14.0% G and 30.6% T. Mitochondrial genome analyses based on NJ method yield phylogenetic trees, including that 20 reported family Ranidae frogs. These molecular data presented here provide a useful tool for systematic analyses of genus .
在本研究中，使用长PCR技术测定了（无尾目：蛙科）的完整线粒体基因组序列。它是一个长度为20571 bp的环状分子（GenBank登录号：MF370348）。该物种的完整线粒体DNA序列包含2个rRNA基因（12S rRNA和16S rRNA）、22个tRNA基因、13个蛋白质编码基因（PCGs）和2个控制区（D环）。核苷酸组成是A占28.6%、C占26.8%、G占14.0%、T占30.6%。基于NJ法的线粒体基因组分析产生了系统发育树，其中包括20种已报道的蛙科蛙类。这里呈现的这些分子数据为该属的系统分析提供了一个有用的工具。
