Fujihara Yuki, Yamanegi Koji, Nagasawa Yasuyuki, Yoshida Ayu, Goto Yukako, Kumanishi Shunsuke, Futani Hiroyuki, Fukunishi Shigeo, Yoshiya Shinichi, Nishiura Hiroshi
Department of Orthopedic Surgery, Hyogo College of Medicine, Nishinomiya, Hyogo, 663-8501, Japan.
Department of Pathology, Hyogo College of Medicine, Nishinomiya, Hyogo, 663-8501, Japan.
Biochem Biophys Rep. 2021 Jan 8;25:100898. doi: 10.1016/j.bbrep.2020.100898. eCollection 2021 Mar.
Circulating lymphocytes infiltrate into local foci at the inflammatory phase of acute wound healing for activation of the immune system and express an immune checkpoint protein programmed cell death 1 (PD-1) at the resolution phase for inactivation of the immune system. Conversely, the PD-1 expression was still found even on circulating lymphocytes of the elder patients with chronic tonsillitis at the palliative stage. Recently, an adhesion G protein coupled receptor 56 (GPR56) was reported to at least work as a proliferation factor for infiltrated lymphocytes into local foci at the resolution phase of acute wound healing. To preliminary examine a similar role of PD-1 and GPR56 at local foci at chronic inflammation, palate tonsils were prepared from small amounts of patients with chronic tonsillitis and tonsillar hypertrophy. A positive relationship of RNA expression might be observed between PD-1 and GPR56 in the elder patients with chronic tonsillitis. In regard to immunohistopathological findings, there were huge and small amounts of PD-1 and GPR56 expression at the marginal zone of lymphoid follicles of palate tonsils with chronic tonsillitis. Moreover, the positive relationship of RNA expression between PD-1 and GPR56 confirmed in large numbers of the elder patients with chronic tonsillitis. Probably, GPR56 participates in a supplement of PD-1 lymphocytes to circulating bloods of the elder patients with chronic tonsillitis through a lymphocyte cell maintenance system at the marginal zone of the lymphoid follicles of palate tonsils.
在急性伤口愈合的炎症阶段，循环淋巴细胞浸润到局部病灶以激活免疫系统，并在愈合阶段表达免疫检查点蛋白程序性细胞死亡1（PD-1）以使免疫系统失活。相反，在姑息阶段，慢性扁桃体炎老年患者的循环淋巴细胞上仍可发现PD-1表达。最近，有报道称粘附G蛋白偶联受体56（GPR56）至少在急性伤口愈合的愈合阶段作为浸润到局部病灶的淋巴细胞的增殖因子发挥作用。为了初步研究PD-1和GPR56在慢性炎症局部病灶中的类似作用，从少量慢性扁桃体炎和扁桃体肥大患者中获取腭扁桃体。在慢性扁桃体炎老年患者中，可能观察到PD-1和GPR56的RNA表达呈正相关。关于免疫组织病理学结果，在患有慢性扁桃体炎的腭扁桃体淋巴滤泡边缘区有大量和少量的PD-1和GPR56表达。此外，在大量慢性扁桃体炎老年患者中证实了PD-1和GPR56的RNA表达呈正相关。可能，GPR56通过腭扁桃体淋巴滤泡边缘区的淋巴细胞维持系统参与向慢性扁桃体炎老年患者循环血液中补充PD-1淋巴细胞。
