在肥大的咽扁桃体中表达程序性死亡受体1（PD-1）及其配体（PD-1L）的CD5CD19 B淋巴细胞的积聚。
Accumulation of CD5CD19 B lymphocytes expressing PD-1 and PD-1L in hypertrophied pharyngeal tonsils.
作者信息
Wlasiuk Paulina, Niedzielski Artur, Skorka Katarzyna, Karczmarczyk Agnieszka, Zaleska Joanna, Zajac Malgorzata, Putowski Maciej, Pac-Kozuchowska Elzbieta, Giannopoulos Krzysztof
机构信息
Experimental Hematooncology Department, Medical University of Lublin, Chodzki 4a, 20093, Lublin, Poland.
Otoneurology Laboratory, Medical University of Lublin, Chodzki 2, 20093, Lublin, Poland.
出版信息
Clin Exp Med. 2016 Nov;16(4):503-509. doi: 10.1007/s10238-015-0385-y. Epub 2015 Aug 29.
Programmed death-1 (PD-1) is one of the most important inhibitory co-receptors expressed predominantly on activated T and B lymphocytes whose expression could be sustained by permanent antigenic stimulation accompanying chronic or recurrent tonsillitis. The expression of PD-1 and PD-1L was analyzed using flow cytometry on hypertrophied tonsils collected from 57 children. We observed high expression of PD-1 and PD-1L on certain lymphocytes subpopulations of hypertrophied tonsils; among T cells, the expression of PD-1 on protein level was higher on CD4 cells (70.3 %) than on CD8 cells (35 %). Interestingly, a limited expression of PD-1 was observed on CD19 B lymphocytes (6.5 %), while CD5CD19 B cells overexpressed PD-1 (52.5 %). Moreover, the expression of PD-1L was also higher on CD5CD19 B cells (16.5 %) than on CD19 B cells (3.5 %) and on CD4 T cells (20 %) than on CD8 T cells (10 %). PD-1 and PD-1L expressions correlated only on CD5CD19 cells. In conclusion, high expression of PD-1 and PD-1L on T and B cells could represent hallmark of immune system adaptation to chronic antigenic exposition in patients with tonsillitis.
程序性死亡受体 1（PD - 1）是最重要的抑制性共受体之一，主要表达于活化的 T 和 B 淋巴细胞上，其表达可由伴随慢性或复发性扁桃体炎的持续性抗原刺激维持。使用流式细胞术分析了从 57 名儿童收集的肥大扁桃体中 PD - 1 和 PD - 1L 的表达。我们观察到肥大扁桃体的某些淋巴细胞亚群上 PD - 1 和 PD - 1L 表达较高；在 T 细胞中，CD4 细胞上 PD - 1 的蛋白水平表达（70.3%）高于 CD8 细胞（35%）。有趣的是，在 CD19 B 淋巴细胞上观察到有限的 PD - 1 表达（6.5%），而 CD5CD19 B 细胞过表达 PD - 1（52.5%）。此外，CD5CD19 B 细胞上 PD - 1L 的表达（16.5%）也高于 CD19 B 细胞（3.5%），且在 CD4 T 细胞上（20%）高于 CD8 T 细胞（10%）。PD - 1 和 PD - 1L 的表达仅在 CD5CD19 细胞上相关。总之，T 和 B 细胞上 PD - 1 和 PD - 1L 的高表达可能代表扁桃体炎患者免疫系统适应慢性抗原暴露的标志。
Zotero 插件