精确控制视觉刺激以研究蝌蚪的经验依赖性神经可塑性。

Precisely controlled visual stimulation to study experience-dependent neural plasticity in tadpoles.

机构信息

The Scripps Research Institute, The Dorris Neuroscience Center, 10550 N. Torrey Pines Rd., San Diego, CA 92037, USA.

出版信息

STAR Protoc. 2021 Jan 8;2(1):100252. doi: 10.1016/j.xpro.2020.100252. eCollection 2021 Mar 19.

DOI:10.1016/j.xpro.2020.100252

PMID:33490972

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7809435/

Abstract

Studies on visual experience-dependent plasticity can benefit tremendously from experimental protocols in which sensory stimulation is precisely controlled for extended periods over which neuronal, circuit, and behavioral plasticity occurs. Small vertebrates, such as tadpoles and zebrafish, are excellent systems for studying brain plasticity. Here, we present a detailed protocol to perform controlled visual stimulation for extended time periods. These methods have been used to study structural plasticity induced by temporally controlled visual stimulation in tadpoles. For further details on the use and execution of this protocol, please refer to Hiramoto and Cline (2014, 2020).

摘要

视觉经验依赖性可塑性的研究可以从实验方案中受益匪浅，这些方案可以精确地控制感官刺激，持续时间长，足以使神经元、电路和行为发生可塑性变化。小型脊椎动物，如蝌蚪和斑马鱼，是研究大脑可塑性的极佳系统。在这里，我们提供了一个详细的方案来进行长时间的受控视觉刺激。这些方法已被用于研究在蝌蚪中由时间控制的视觉刺激引起的结构可塑性。有关此方案的使用和执行的更多详细信息，请参见 Hiramoto 和 Cline（2014 年，2020 年）。