Department of Surgery, University of Wisconsin, Madison, WI 53705, USA.
Department of Orthopedics, University of Wisconsin, Madison, WI 53705, USA.
STAR Protoc. 2021 Jan 13;2(1):100261. doi: 10.1016/j.xpro.2020.100261. eCollection 2021 Mar 19.
Pericytes play a critical role in promoting, regulating, and maintaining numerous vascular functions. Their dysfunction is a major contributor to the progression of vascular and neurodegenerative diseases, making them an ideal candidate for large-scale production for disease modeling and regenerative cell therapy. This protocol describes the rapid and robust differentiation of pericytes from human induced pluripotent stem cells (hiPSCs) while simultaneously generating a population of hiPSC-derived endothelial progenitor cells. For complete details on the use and execution of this protocol, please refer to Zhang et al. (2017).
周细胞在促进、调节和维持多种血管功能方面发挥着关键作用。它们的功能障碍是血管和神经退行性疾病进展的主要原因，这使得它们成为用于疾病建模和再生细胞治疗的大规模生产的理想候选物。本方案描述了如何从人诱导多能干细胞（hiPSC）中快速而稳健地分化出周细胞，同时生成一群 hiPSC 衍生的内皮祖细胞。有关使用和执行本方案的完整详细信息，请参阅 Zhang 等人（2017 年）。
