NIMBE, CEA, CNRS, Université Paris-Saclay, CEA Saclay, Gif-sur-Yvette, France.
Centre de Résonance Magnétique des Systèmes Biologiques, CNRS-Université de Bordeaux, UMR5536, Bordeaux, France.
NMR Biomed. 2021 Apr;34(4):e4477. doi: 10.1002/nbm.4477. Epub 2021 Jan 24.
This study explores the potential of profiling a microgram-scale soft tissue biopsy by NMR spectroscopy. The important elements of high resolution and high sensitivity for the spectral data are achieved through a unique probe, HR-μMAS, which allowed comprehensive profiling to be performed on microgram tissue for the first time under MAS conditions. Thorough spatially resolved metabolic maps were acquired across a coronal brain slice of rat C6 gliomas, which rendered the delineation of the tumor lesion. The results present a unique ex vivo NMR possibility to analyze tissue pathology that cannot be fully explored by the conventional approach, HR-MAS and in vivo MRS. Aside from the capability of analyzing a small localized region to track its specific metabolism, it could also offer the possibility to carry out longitudinal investigations on live animals due to the feasibility of minimally invasive tissue excision.
本研究探讨了通过 NMR 光谱对微克级软组织活检进行分析的可能性。通过独特的探头 HR-μMAS，实现了光谱数据的高分辨率和高灵敏度，这使得首次能够在 MAS 条件下对微克级组织进行全面分析。在大鼠 C6 神经胶质瘤的冠状脑切片上获得了详尽的空间分辨代谢图谱，从而描绘出肿瘤病变。结果表明，NMR 具有独特的离体可能性，可以分析组织病理学，而这是传统方法 HR-MAS 和体内 MRS 无法完全探索的。除了能够分析小的局部区域以跟踪其特定代谢的能力外，由于微创组织切除的可行性，它还可以为对活体动物进行纵向研究提供可能性。
