University of Chinese Academy of Sciences, Beijing 100049, PR China.
CAS Key Laboratory of Urban Pollutant Conversion，Fujian Key Laboratory of Watershed Ecology, Institute of Urban Environment, Chinese Academy of Sciences, Xiamen, Fujian 361021, PR China.
Int J Syst Evol Microbiol. 2021 Feb;71(2). doi: 10.1099/ijsem.0.004658.
A bisphenol A-degrading bacterium, designated as strain H4, was isolated from surface seawater, which was sampled from the Jiulong River estuary in southeast PR China. Strain H4 is Gram-stain-negative, aerobic, short rod-shaped, lacking bacteriochlorophyll a, motile with multifibrillar stalklike fascicle structures and capable of degrading bisphenol A. Growth of strain H4 was observed at 24-45 °C (optimum, 32 °C), at pH 5.5-9 (optimum, pH 7.0) and in 0-7 % NaCl (optimum, 2 %; w/v) . The 16S rRNA gene sequence of strain H4 showed highest similarity to Ery9 (98.7 %), (98.3 %), PQ-2 (98.1 %) and GM-16 (97.6 %), followed by E4A9 (96.7 %) and Ery22 (96.0 %). Phylogenetic analysis revealed that strain H4 fell within a clade comprising the type strains of species and formed a phyletic line with them that was distinct from other members of the family . The sole respiratory quinone was quinone 10 (Q-10). The predominant fatty acids (>5 % of the total fatty acids) of strain H4 were summed feature 8 (C 6 and/or C 7), summed feature 3 (C 6 and/or C 7), C 6 and C2-OH. The genomic DNA G+C content was 62.8 mol%. In the polar lipid profile, phosphatidylcholine, phosphatidylethanolamine, phosphatidylglycerol, two unidentified phospholipids, two sphingoglycolipids and three unknown lipids were the major compounds. Based on the genotypic and phenotypic data, strain H4 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is H4 (=DSM 102182=MCCC1 K02301).
一株可降解双酚 A 的细菌，被命名为 H4 菌株，从中国东南部九龙江河口的海水表面分离得到。H4 菌株为革兰氏阴性、需氧、短杆状、缺乏细菌叶绿素 a、具有多纤维束状束状结构的运动性，并能降解双酚 A。H4 菌株的生长在 24-45°C（最佳，32°C）、pH 5.5-9（最佳，pH 7.0）和 0-7%NaCl（最佳，2%；w/v）之间。H4 菌株的 16S rRNA 基因序列与 Ery9（98.7%）、（98.3%）、PQ-2（98.1%）和 GM-16（97.6%）的相似度最高，其次是 E4A9（96.7%）和 Ery22（96.0%）。系统发育分析表明，H4 菌株属于一个包含 Ery 属模式菌株的分支，并与它们形成一个独特的分支，与该属的其他成员不同。唯一的呼吸醌是醌 10（Q-10）。H4 菌株的主要脂肪酸（占总脂肪酸的>5%）是总和特征 8（C6 和/或 C7）、总和特征 3（C6 和/或 C7）、C6 和 C2-OH。基因组 DNA G+C 含量为 62.8mol%。在极性脂谱中，磷脂酰胆碱、磷脂酰乙醇胺、磷脂酰甘油、两种未鉴定的磷脂、两种神经酰胺糖脂和三种未知脂质是主要化合物。基于基因型和表型数据，H4 菌株代表了一个新的 Ery 属物种，命名为 sp. nov.。模式菌株为 H4（=DSM 102182=MCCC1 K02301）。
