Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application, Key Laboratory of Agricultural Microbiomics and Precision Application, Ministry of Agriculture and Rural Affairs, State Key Laboratory of Applied Microbiology Southern China, Guangdong Microbial Culture Collection Center (GDMCC), Institute of Microbiology, Guangdong Academy of Sciences, Building 66, Xianlie Middle Road 100, Guangzhou, 510070, Guangdong Province, People's Republic of China.
Technical Service Department, Fuzhoushi Shanhu Feed CO., LTD, Guangdong Evergreen Feed Industry CO., LTD, Zhanjiang, 524022, People's Republic of China.
Arch Microbiol. 2021 Dec 28;204(1):93. doi: 10.1007/s00203-021-02703-x.
A novel Gram-staining-negative and short-rod-shaped bacterial strain designated as 1NDH52 was isolated from a tidal flat sediment and characterized using a polyphasic taxonomic approach. The predominant cellular fatty acids of strain 1NDH52 were summed feature 8 (C ω7c and/or C ω6c) and C 2-OH; the major polar lipids were diphosphatidylglycerol, phosphatidylcholine, phosphatidylethanolamine, phosphatidylglycerol and sphingoglycolipid; the major respiratory quinone was Q-10. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain 1NDH52 was closely related to type strains Croceicoccus sediminis S2-4-2 (98.9%), Croceicoccus bisphenolivorans H4 (98.9%) and Croceicoccus pelagius Ery9 (98.7%). Phylogenomic analysis indicated that strain 1NDH52 formed an independent branch distinct from all type strains of this genus. The overall genome related indices including the digital DNA-DNA hybridization values, average nucleotide identities and average amino acid identities between strain 1NDH52 and the three close relatives above indicated that strain 1NDH52 should represent a novel genospecies. The genomic DNA G + C content was 62.6%. Strain 1NDH52 could produce carotenoids and its genome contained the complete carotenoids biosynthetic gene cluster. Based on the phenotypic and genotypic characteristics, strain 1NDH52 is concluded to represent a novel species of the genus Croceicoccus, for which the name Croceicoccus gelatinilyticus sp. nov., is proposed. The type strain of the species is 1NDH52 (= GDMCC 1.2381 = KCTC 82668).
一株新的革兰氏阴性短杆菌菌株 1NDH52 是从潮滩沉积物中分离出来的，采用多相分类学方法进行了鉴定。菌株 1NDH52 的主要细胞脂肪酸为特征 8（C ω7c 和/或 C ω6c）和 C 2-OH；主要极性脂为双磷脂酰甘油、磷脂酰胆碱、磷脂酰乙醇胺、磷脂酰甘油和神经酰胺糖脂；主要呼吸醌为 Q-10。基于 16S rRNA 基因序列的系统发育分析表明，菌株 1NDH52 与模式菌株 Croceicoccus sediminis S2-4-2（98.9%）、Croceicoccus bisphenolivorans H4（98.9%）和 Croceicoccus pelagius Ery9（98.7%）密切相关。基因组分析表明，菌株 1NDH52 形成了一个与该属所有模式菌株都不同的独立分支。菌株 1NDH52 与上述三个近缘种之间的全基因组相关指数，包括数字 DNA-DNA 杂交值、平均核苷酸同一性和平均氨基酸同一性，表明菌株 1NDH52 应代表一个新的种。基因组 DNA G+C 含量为 62.6%。菌株 1NDH52 可产生类胡萝卜素，其基因组包含完整的类胡萝卜素生物合成基因簇。基于表型和基因型特征，菌株 1NDH52 代表 Croceicoccus 属的一个新种，命名为 Croceicoccus gelatinilyticus sp. nov.。该种的模式菌株为 1NDH52（=GDMCC 1.2381=KCTC 82668）。
