Croceicoccus gelatinilyticus sp. nov., isolated from a tidal flat sediment.

A novel Gram-staining-negative and short-rod-shaped bacterial strain designated as 1NDH52 was isolated from a tidal flat sediment and characterized using a polyphasic taxonomic approach. The predominant cellular fatty acids of strain 1NDH52 were summed feature 8 (C ω7c and/or C ω6c) and C 2-OH; the major polar lipids were diphosphatidylglycerol, phosphatidylcholine, phosphatidylethanolamine, phosphatidylglycerol and sphingoglycolipid; the major respiratory quinone was Q-10. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain 1NDH52 was closely related to type strains Croceicoccus sediminis S2-4-2 (98.9%), Croceicoccus bisphenolivorans H4 (98.9%) and Croceicoccus pelagius Ery9 (98.7%). Phylogenomic analysis indicated that strain 1NDH52 formed an independent branch distinct from all type strains of this genus. The overall genome related indices including the digital DNA-DNA hybridization values, average nucleotide identities and average amino acid identities between strain 1NDH52 and the three close relatives above indicated that strain 1NDH52 should represent a novel genospecies. The genomic DNA G + C content was 62.6%. Strain 1NDH52 could produce carotenoids and its genome contained the complete carotenoids biosynthetic gene cluster. Based on the phenotypic and genotypic characteristics, strain 1NDH52 is concluded to represent a novel species of the genus Croceicoccus, for which the name Croceicoccus gelatinilyticus sp. nov., is proposed. The type strain of the species is 1NDH52 (= GDMCC 1.2381 = KCTC 82668).