Lok E, Nera E A, Iverson F, Scott F, So Y, Clayson D B
Toxicology Research Division, Bureau of Chemical Safety, Health & Welfare, Canada, Ottawa, Ontario.
Cancer Lett. 1988 Jan;38(3):249-55. doi: 10.1016/0304-3835(88)90016-x.
Four groups of female Swiss Webster mice were given either laboratory chow or a purified (semi-synthetic) diet (AIN-76A) either ad libitum or at 75% of the ad libitum rate for about 30 days. Three tissues, the crypt cells of the jejunum, the dermis and the basal epithelial cells of the esophagus were investigated using [3H]thymidine labelling and by counting mitoses; four other tissues, the alveolar cells of the mammary gland, the crypt cells of the duodenum and colo-rectum, and the transitional cells of the urinary bladder were examined using [3H]thymidine labelling only. In each case dietary restriction led to a reduction of cellular proliferation assessed by these indices. The potential of the approach for the study of the effects of dietary modification on the induction of cancer is discussed.
将四组雌性瑞士韦伯斯特小鼠分为两组，分别给予实验室常规饲料或纯化（半合成）饲料（AIN - 76A），一组自由采食，另一组按自由采食量的75%喂食，持续约30天。使用[³H]胸腺嘧啶核苷标记并计数有丝分裂，对三组组织（空肠隐窝细胞、真皮和食管基底上皮细胞）进行了研究；另外四组组织（乳腺肺泡细胞、十二指肠和结肠直肠隐窝细胞以及膀胱移行细胞）仅使用[³H]胸腺嘧啶核苷标记进行了检查。在每种情况下，通过这些指标评估，饮食限制均导致细胞增殖减少。本文还讨论了该方法在研究饮食改变对癌症诱导影响方面的潜力。
