Department of Chemistry, Chungbuk National University, Cheongju 28644, Korea.
Molecules. 2021 Jan 21;26(3):556. doi: 10.3390/molecules26030556.
The expanding scope of chemical reactions applied to nucleic acids has diversified the design of nucleic acid-based technologies that are essential to medicinal chemistry and chemical biology. Among chemical reactions, visible light photochemical reaction is considered a promising tool that can be used for the manipulations of nucleic acids owing to its advantages, such as mild reaction conditions and ease of the reaction process. Of late, inspired by the development of visible light-absorbing molecules and photocatalysts, visible light-driven photochemical reactions have been used to conduct various molecular manipulations, such as the cleavage or ligation of nucleic acids and other molecules as well as the synthesis of functional molecules. In this review, we describe the recent developments (from 2010) in visible light photochemical reactions involving nucleic acids and their applications in the design of nucleic acid-based technologies including DNA photocleaving, DNA photoligation, nucleic acid sensors, the release of functional molecules, and DNA-encoded libraries.
应用于核酸的化学反应范围不断扩大，使基于核酸的技术的设计多样化，这些技术对药物化学和化学生物学至关重要。在化学反应中，可见光光化学反应因其反应条件温和、反应过程简便等优点，被认为是一种很有前途的核酸操作工具。近年来，受可见光吸收分子和光催化剂发展的启发，可见光驱动的光化学反应已被用于进行各种分子操作，如核酸和其他分子的断裂或连接以及功能分子的合成。在本文中，我们描述了近年来（2010 年以来）涉及核酸的可见光光化学反应的最新进展及其在基于核酸的技术设计中的应用，包括 DNA 光裂解、DNA 光连接、核酸传感器、功能分子的释放和 DNA 编码文库。
