Department of Cellular and Molecular Medicine, University of California, San Diego, La Jolla, California.
Glycobiology Research and Training Center, University of California, San Diego, La Jolla, California.
J Histochem Cytochem. 2021 Feb;69(2):105-119. doi: 10.1369/0022155420988661.
Heparan sulfate proteoglycans consist of a small family of proteins decorated with one or more covalently attached heparan sulfate glycosaminoglycan chains. These chains have intricate structural patterns based on the position of sulfate groups and uronic acid epimers, which dictate their ability to engage a large repertoire of heparan sulfate-binding proteins, including extracellular matrix proteins, growth factors and morphogens, cytokines and chemokines, apolipoproteins and lipases, adhesion and growth factor receptors, and components of the complement and coagulation system. This review highlights recent progress in the characterization of the so-called "heparan sulfate interactome," with a major focus on systems-wide strategies as a tool for discovery and characterization of this subproteome. In addition, we compiled all heparan sulfate-binding proteins reported in the literature to date and grouped them into a few major functional classes by applying a networking approach.
硫酸乙酰肝素蛋白聚糖由一小家族蛋白组成，这些蛋白通过共价结合的硫酸乙酰肝素糖胺聚糖链进行修饰。这些链具有复杂的结构模式，基于硫酸基团和糖醛酸差向异构体的位置，决定了它们与大量硫酸乙酰肝素结合蛋白相互作用的能力，包括细胞外基质蛋白、生长因子和形态发生素、细胞因子和趋化因子、载脂蛋白和脂肪酶、黏附生长因子受体以及补体和凝血系统的成分。这篇综述强调了最近在所谓的“硫酸乙酰肝素相互作用组”的特征描述方面的进展，主要侧重于系统范围的策略，作为发现和描述这个亚蛋白组的工具。此外，我们还收集了迄今为止文献中报道的所有硫酸乙酰肝素结合蛋白，并通过应用网络方法将它们分为几个主要的功能类别。
