奥氮平作为一种抗精神病药物对神经胶质瘤细胞的放射增敏作用。
The Radiosensitizing Effect of Olanzapine as an Antipsychotic Medication on Glioblastoma Cell.
机构信息
Department of Radiopharmacy, Faculty of Pharmacy, Pharmaceutical Sciences Research Center, Mazandaran University of Medical Sciences, Sari, Iran.
Department of Radiology and Radiation Oncology, Faculty of Medicine, Mazandaran University of Medical Sciences, Sari, Iran.
出版信息
Curr Radiopharm. 2022;15(1):50-55. doi: 10.2174/1874471014666210120100448.
BACKGROUND
Radiotherapy is used as one of the most effective regimens for cancer treatment, while radioresistance is a major drawback in cancer treatment.
OBJECTIVES
This study aimed to evaluate the sensitizing effect of olanzapine (OLA) with X-ray on glioblastoma (U-87 MG) cells death.
METHODS
The synergistic killing effect of OLA with ionizing radiation (IR) on glioma was evaluated by colony formation assay. The generations of reactive oxygen species (ROS) and protein carbonyl (PC) as oxidized proteins were determined in OLA-treated and irradiated cells.
RESULTS
Results of this study showed that OLA reduced the number of colonies in irradiated glioma cells.OLA elevated ROS and PC levels in irradiated cells. The synergistic killing effect of OLA with IR in U-87 MG cells was observed at concentrations of 1 μM and 20 μM of OLA. The maximum radiosensitizing effect of OLA was observed at a concentration of 20 μM.
CONCLUSION
The present study demonstrates that OLA has a radiosensitizing effect on cell death induced by IR in glioma cells.
背景
放射疗法被用作癌症治疗最有效的方案之一，而放射抗性是癌症治疗的主要障碍。
目的
本研究旨在评估奥氮平（OLA）与 X 射线联合对神经胶质瘤（U-87 MG）细胞死亡的敏化作用。
方法
通过集落形成实验评估 OLA 与电离辐射（IR）对神经胶质瘤的协同杀伤作用。在 OLA 处理和辐照的细胞中测定活性氧（ROS）和蛋白质羰基（PC）的产生，作为氧化蛋白。
结果
本研究结果表明，OLA 减少了辐照神经胶质瘤细胞中的菌落数量。OLA 增加了辐照细胞中的 ROS 和 PC 水平。在浓度为 1 μM 和 20 μM 的 OLA 时观察到 OLA 与 IR 在 U-87 MG 细胞中的协同杀伤作用。OLA 的最大放射增敏作用在浓度为 20 μM 时观察到。
结论
本研究表明，OLA 对 IR 诱导的神经胶质瘤细胞死亡具有放射增敏作用。
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