Tan Lei, Zhang Yuqiang, Liu Fang, Yuan Yanmei, Zhan Yuan, Sun Yingjie, Qiu Xusheng, Meng Chunchun, Song Cuiping, Ding Chan
Department of Avian Diseases, Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai 200241, PR China.
Department of Avian Diseases, Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai 200241, PR China; Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou 225009, PR China.
Vaccine. 2016 Oct 17;34(44):5209-5216. doi: 10.1016/j.vaccine.2016.09.022. Epub 2016 Sep 21.
Infectious bronchitis virus (IBV) causes major losses in the poultry industry. The safe and effective vaccine to control IBV spread is imperative. In the present study, we developed IBV S1 glycoprotein poly-epitope-based DNA vaccine pV-S1B+S1T consisting of SH1208 and Holte strain BF2-restricted T cell epitopes and Australian T strain dominant B cell neutralization epitopes. Specific pathogen-free chickens were vaccinated with pV-S1B+S1T and control plasmids twice to elicit strong humoral and cellular immune response, as indicated by viral neutralization titers and results of CD8 T cell proliferation assays. A lethal dose of IBV SH1208 strain used for protection and challenge experiments at two weeks post-booster immunization following challenge protection and virus shedding reverse transcription quantitative PCR assay, indicated that pV-S1B+S1T protected against IBV and significantly reduced viral excretion. These results demonstrated that the IBV poly-epitope-based vaccine effectively prevents infection and represents a potential IBV vaccine.
传染性支气管炎病毒（IBV）给家禽业造成重大损失。控制IBV传播的安全有效疫苗势在必行。在本研究中，我们开发了基于IBV S1糖蛋白多表位的DNA疫苗pV-S1B+S1T，其由SH1208和Holte株BF2限制性T细胞表位以及澳大利亚T株优势B细胞中和表位组成。用pV-S1B+S1T和对照质粒对无特定病原体鸡进行两次接种，以引发强烈的体液和细胞免疫反应，病毒中和滴度和CD8 T细胞增殖试验结果表明了这一点。在加强免疫两周后，使用致死剂量的IBV SH1208株进行保护和攻毒实验，攻毒保护和病毒排毒逆转录定量PCR检测结果表明，pV-S1B+S1T可预防IBV感染，并显著减少病毒排泄。这些结果表明，基于IBV多表位的疫苗能有效预防感染，是一种有潜力的IBV疫苗。
