Department of Veterinary Public Health and Food Safety, Faculty of Veterinary Medicine, Ghent University, Salisburylaan 133, 9820, Merelbeke, Belgium.
Laboratory of Microbiology, Department of Biochemistry and Microbiology, Ghent University, Karel Lodewijk Ledeganckstraat 35, 9000, Ghent, Belgium.
Food Microbiol. 2021 Jun;96:103724. doi: 10.1016/j.fm.2020.103724. Epub 2020 Dec 31.
Proteus mirabilis is an important pathogen involved in human urinary tract infections, and also more isolated from stools of patients with diarrheal disease than from healthy patients. The role of food, especially poultry products as source for human infection and multi-resistant strains remains unclear. As a resident in broilers' intestines, P. mirabilis can contaminate broiler carcasses due to slaughter practices, and be a risk for human infection. The present study evaluated the performance of five isolation media, and subsequently examined the presence of P. mirabilis on broiler carcasses at retail. Additionally, isolates were characterized by the Dienes' test, repetitive element PCR fingerprinting and pulsed-field gel electrophoresis, and their antibiotic resistance profile determined. Using a combined isolation protocol on blood agar, xylose lysine deoxycholate agar and violet red bile glucose agar, P. mirabilis was isolated from 29 out of 80 broiler carcasses (36.25%) with a mean contamination level of 2.25 ± 0.50 log CFU/g. A high strain heterogeneity was present in isolates from broilers and human stool. The same strains were not shared, but the antibiotic resistance profiling was similar. A role of poultry products as source for human infection should be taken into account.
奇异变形杆菌是一种重要的人类尿路感染病原体，且比健康患者的粪便中更常分离到奇异变形杆菌。食物，尤其是家禽产品作为人类感染和多耐药菌株的来源的作用尚不清楚。奇异变形杆菌作为肉鸡肠道的居民，由于屠宰操作，可能会污染肉鸡胴体，并对人类感染构成威胁。本研究评估了五种分离培养基的性能，随后检查了零售肉鸡胴体上奇异变形杆菌的存在情况。此外，还通过 Dienes 试验、重复元件 PCR 指纹图谱和脉冲场凝胶电泳对分离株进行了特征描述，并确定了它们的抗生素耐药谱。采用血液琼脂、木糖赖氨酸去氧胆酸盐琼脂和紫色红胆葡萄糖琼脂的联合分离方案，从 80 只肉鸡胴体中分离出 29 株奇异变形杆菌（36.25%），平均污染水平为 2.25±0.50 log CFU/g。从肉鸡和人类粪便中分离出的菌株存在高度的菌株异质性。没有共享相同的菌株，但抗生素耐药谱相似。应考虑将家禽产品作为人类感染的来源。
