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UVR8与从头DNA甲基转移酶相互作用并抑制拟南芥中的DNA甲基化。

UVR8 interacts with de novo DNA methyltransferase and suppresses DNA methylation in Arabidopsis.

作者信息

Jiang Jianjun, Liu Jie, Sanders Dean, Qian Shuiming, Ren Wendan, Song Jikui, Liu Fengquan, Zhong Xuehua

机构信息

Institute of Plant Protection, Jiangsu Academy of Agricultural Sciences, Jiangsu Key Laboratory for Food Quality and Safety-State Key Laboratory Cultivation Base of Ministry of Science and Technology, Nanjing, Jiangsu, China.

Laboratory of Genetics & Wisconsin Institute for Discovery, University of Wisconsin-Madison, Madison, WI, USA.

出版信息

Nat Plants. 2021 Feb;7(2):184-197. doi: 10.1038/s41477-020-00843-4. Epub 2021 Jan 25.

DOI:10.1038/s41477-020-00843-4
PMID:33495557
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7889724/
Abstract

DNA methylation is an important epigenetic gene regulatory mechanism conserved in eukaryotes. Emerging evidence shows DNA methylation alterations in response to environmental cues. However, the mechanism of how cells sense these signals and reprogramme the methylation landscape is poorly understood. Here, we uncovered a connection between ultraviolet B (UVB) signalling and DNA methylation involving UVB photoreceptor (UV RESISTANCE LOCUS 8 (UVR8)) and a de novo DNA methyltransferase (DOMAINS REARRANGED METHYLTRANSFERASE 2 (DRM2)) in Arabidopsis. We demonstrated that UVB acts through UVR8 to inhibit DRM2-mediated DNA methylation and transcriptional de-repression. Interestingly, DNA transposons with high DNA methylation are more sensitive to UVB irradiation. Mechanistically, UVR8 interacts with and negatively regulates DRM2 by preventing its chromatin association and inhibiting the methyltransferase activity. Collectively, this study identifies UVB as a potent inhibitor of DNA methylation and provides mechanistic insights into how signalling transduction cascades intertwine with chromatin to guide genome functions.

摘要

DNA甲基化是真核生物中一种重要的表观遗传基因调控机制。新出现的证据表明，DNA甲基化会因环境线索而发生改变。然而，细胞如何感知这些信号并重新编程甲基化图谱的机制仍知之甚少。在这里，我们发现了拟南芥中紫外线B（UVB）信号与DNA甲基化之间的联系，涉及UVB光感受器（抗紫外线位点8（UVR8））和一种从头DNA甲基转移酶（结构域重排甲基转移酶2（DRM2））。我们证明，UVB通过UVR8发挥作用，抑制DRM2介导的DNA甲基化和转录去抑制。有趣的是，具有高DNA甲基化的DNA转座子对UVB照射更敏感。从机制上讲，UVR8与DRM2相互作用并通过阻止其与染色质结合并抑制甲基转移酶活性来负向调节DRM2。总的来说，这项研究确定UVB是DNA甲基化的有效抑制剂，并提供了信号转导级联如何与染色质相互交织以指导基因组功能的机制见解。

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3
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