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小麦中的晚熟α-淀粉酶及其对新鲜白酱品质的影响。

Late-Maturity Alpha-Amylase in Wheat () and Its Impact on Fresh White Sauce Qualities.

作者信息

Neoh Galex K S, Dieters Mark J, Tao Keyu, Fox Glen P, Nguyen Phuong T M, Gilbert Robert G

机构信息

Joint International Research Laboratory of Agriculture and Agri-Product Safety, College of Agriculture, Yangzhou University, Yangzhou 225009, China.

Centre for Nutrition and Food Science, Queensland Alliance for Agriculture and Food Innovation (QAAFI), The University of Queensland, Brisbane, QLD 4072, Australia.

出版信息

Foods. 2021 Jan 20;10(2):201. doi: 10.3390/foods10020201.

DOI:10.3390/foods10020201
PMID:33498449
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7909430/
Abstract

When wheat experiences a cold-temperature 'shock' during the late stage of grain filling, it triggers the abnormal synthesis of late-maturity -amylase (LMA). This increases the enzyme content in affected grain, which can lead to a drastic reduction in falling number (FN). By commercial standards, a low FN is taken as an indication of inferior quality, deemed unsuitable for end-product usage. Hence, LMA-affected grains are either rejected or downgraded to feed grade at the grain receiving point. However, previous studies have found no substantial correlation between low FN-LMA and bread quality. The present study extends previous investigations to semi-solid food, evaluating the physical quality of fresh white sauce processed from LMA-affected flour. Results show that high-LMA flours had low FNs and exhibited poor pasting characteristics. However, gelation occurred in the presence of other components during fresh white sauce processing. This demonstrates that LMA-affected flours may have new applications in low-viscosity products.

摘要

当小麦在灌浆后期经历低温“冲击”时,会引发晚期成熟α-淀粉酶(LMA)的异常合成。这会增加受影响籽粒中的酶含量,进而可能导致降落数值(FN)急剧降低。按照商业标准,低FN被视为质量较差的指标,被认为不适用于最终产品用途。因此,受LMA影响的籽粒在谷物接收点要么被拒收,要么被降级为饲料级。然而,先前的研究发现低FN-LMA与面包质量之间没有实质性关联。本研究将先前的调查扩展到半固体食品,评估了用受LMA影响的面粉加工的新鲜白酱的物理质量。结果表明,高LMA面粉的FN较低,且呈现出较差的糊化特性。然而,在新鲜白酱加工过程中,在其他成分存在的情况下会发生凝胶化。这表明受LMA影响的面粉可能在低粘度产品中有新的应用。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f911/7909430/76f2179a3d14/foods-10-00201-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f911/7909430/2000423a450a/foods-10-00201-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f911/7909430/a81ba6b8e589/foods-10-00201-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f911/7909430/c6b400d6f6f0/foods-10-00201-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f911/7909430/76f2179a3d14/foods-10-00201-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f911/7909430/2000423a450a/foods-10-00201-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f911/7909430/a81ba6b8e589/foods-10-00201-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f911/7909430/c6b400d6f6f0/foods-10-00201-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f911/7909430/76f2179a3d14/foods-10-00201-g004.jpg

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本文引用的文献

1
Impacts of Starch and the Interactions Between Starch and Other Macromolecules on Wheat Falling Number.淀粉及其与其他大分子之间的相互作用对小麦降落数值的影响
Compr Rev Food Sci Food Saf. 2019 May;18(3):641-654. doi: 10.1111/1541-4337.12430. Epub 2019 Mar 28.
2
Evaluation of the impact of heat on wheat dormancy, late maturity α-amylase and grain size under controlled conditions in diverse germplasm.评价控制条件下不同种质资源小麦耐热休眠、晚熟 α-淀粉酶和粒重的影响。
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Late-maturity α-amylase expression in wheat is influenced by genotype, temperature and stage of grain development.
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Gigascience. 2022 Dec 28;12. doi: 10.1093/gigascience/giad084. Epub 2023 Nov 1.
小麦中晚期 α-淀粉酶的表达受基因型、温度和籽粒发育阶段的影响。
Planta. 2020 Jan 16;251(2):51. doi: 10.1007/s00425-020-03341-1.
4
Does Late Maturity Alpha-Amylase Impact Wheat Baking Quality?晚熟α-淀粉酶会影响小麦烘焙品质吗?
Front Plant Sci. 2018 Sep 7;9:1356. doi: 10.3389/fpls.2018.01356. eCollection 2018.
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Impact of Preharvest Sprouting of Wheat (Triticum aestivum) in the Field on Starch, Protein, and Arabinoxylan Properties.田间小麦(普通小麦)收获前发芽对淀粉、蛋白质和阿拉伯木聚糖特性的影响
J Agric Food Chem. 2016 Nov 9;64(44):8324-8332. doi: 10.1021/acs.jafc.6b03140. Epub 2016 Oct 25.
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Wheat grain preharvest sprouting and late maturity alpha-amylase.小麦收获前发芽和晚熟α-淀粉酶。
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Engineering α-amylase levels in wheat grain suggests a highly sophisticated level of carbohydrate regulation during development.调控小麦籽粒中的α-淀粉酶水平表明其在发育过程中碳水化合物调节水平高度复杂。
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Α-amylase from wheat (Triticum aestivum) seeds: its purification, biochemical attributes and active site studies.小麦(Triticum aestivum)种子中的α-淀粉酶:其纯化、生化特性和活性位点研究。
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