López-Abán Julio, Vicente Belén, Kabbas-Piñango Elías, Hernández-Goenaga Juan, Sánchez-Montejo Javier, Aguiriano María, Del Olmo Esther, Vanegas Magnolia, Patarroyo Manuel Alfonso, Muro Antonio
Infectious and Tropical Diseases Group (e-INTRO), IBSAL-CIETUS (Biomedical Research Institute of Salamanca-Research Centre for Tropical Diseases at the University of Salamanca), Faculty of Pharmacy, University of Salamanca, Ldo Méndez Nieto s/n, 37007 Salamanca, Spain.
Department of Pharmaceutical Chemistry, IBSAL-CIETUS, Faculty of Pharmacy, University of Salamanca, Ldo Méndez Nieto s/n, 37007 Salamanca, Spain.
J Clin Med. 2021 Jan 24;10(3):445. doi: 10.3390/jcm10030445.
Schistosomiasis is a parasitic disease that affects 143 million people in endemic countries. This work analyzed overexpressed sequences from the cercaria phase to the early schistosomulum phase using bioinformatics tools to predict host interaction and selected proteins for predicting T cell epitopes. The final peptides were chemically synthesized, and their toxicity was evaluated in vitro. Peptides were formulated in the Adjuvant Adaptation (ADAD) vaccination system and injected into BALB/c mice that were challenged with cercariae to assess protection and immunogenicity. A total of 39 highly expressed proteins were identified as being of potential interest. Three T cell peptides predicted to bind MHC mouse and human class II were synthesized and formulated for vaccination. SmGSP and SmIKE reduced the number of eggs trapped in the liver by more than 50% in challenged BALB/c mice. The liver of mice vaccinated with either SmGSP or SmTNP had a significantly reduced affected liver surface. Transcriptome-based T cell peptides elicit partial protection and could be candidates for a multiantigen vaccine.
血吸虫病是一种寄生虫病，在流行国家影响着1.43亿人。这项工作使用生物信息学工具分析了从尾蚴阶段到早期童虫阶段的过表达序列，以预测宿主相互作用，并选择用于预测T细胞表位的蛋白质。最终的肽段通过化学合成，并在体外评估其毒性。肽段被配制在佐剂适应性（ADAD）疫苗接种系统中，并注射到用尾蚴攻击的BALB/c小鼠体内，以评估其保护性和免疫原性。总共鉴定出39种高表达的潜在感兴趣蛋白质。合成并配制了三种预测可与MHC小鼠和人类II类结合的T细胞肽用于疫苗接种。在受到攻击的BALB/c小鼠中，SmGSP和SmIKE使肝脏中捕获的虫卵数量减少了50%以上。接种SmGSP或SmTNP的小鼠肝脏受影响的表面明显减少。基于转录组的T细胞肽可引发部分保护作用，可能成为多抗原疫苗的候选物。
