Villatoro Antonio J, Martín-Astorga María Del Carmen, Alcoholado Cristina, Sánchez-Martín María Del Mar, Becerra José
Laboratory of Bioengineering and Tissue Regeneration, Department of Cell Biology, Genetics and Physiology, University of Málaga, IBIMA, 29071 Málaga, Spain.
Instituto de Immunología Clínica y Terapia Celular (IMMUNESTEM), 29071 Málaga, Spain.
Animals (Basel). 2021 Jan 24;11(2):295. doi: 10.3390/ani11020295.
Mesenchymal stem cells (MSCs) have been shown to have therapeutic efficacy in different complex pathologies in feline species. This effect is attributed to the secretion of a wide variety of bioactive molecules and extracellular vesicles, such as exosomes, with significant paracrine activity, encompassed under the concept of the secretome. However, at present, the exosomes from feline MSCs have not yet been studied in detail. The objective of this study is to analyze and compare the protein profiles of the secretome as a whole and its exosomal fraction from feline adipose-derived MSCs (fAd-MSCs). For this, Gene Ontology (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG) and Protein-Protein Interaction Networks Functional Enrichment Analysis (STRING) were utilized. A total of 239 proteins were identified in the secretome, and 228 proteins specific to exosomes were identified, with a total of 133 common proteins. The proteins identified in the secretome were located in the extracellular regions and in the cytoplasm, while the exosomal proteins were located mainly in the membrane, cytoplasm and cytosol. Regarding function, in the secretome, proteins involved in different metabolic pathways, in pathways related to the immune system and the endocrine system and in the processing of proteins in the endoplasmic reticulum predominated. In contrast, proteins specific to exosomes were predominantly associated with endocytosis, cell junctions, platelet activation and other cell signaling pathways. The possible future use of the secretome, or some of its components, such as exosomes, would provide a non-cell-based therapeutic strategy for the treatment of different diseases that would avoid the drawbacks of cell therapy.
间充质干细胞（MSCs）已被证明在猫科动物的不同复杂病症中具有治疗效果。这种作用归因于多种生物活性分子和细胞外囊泡的分泌，比如具有显著旁分泌活性的外泌体，这些都包含在分泌组的概念之下。然而，目前猫科动物间充质干细胞来源的外泌体尚未得到详细研究。本研究的目的是分析和比较猫脂肪来源的间充质干细胞（fAd-MSCs）分泌组整体及其外泌体部分的蛋白质谱。为此，使用了基因本体论（GO）、京都基因与基因组百科全书（KEGG）以及蛋白质-蛋白质相互作用网络功能富集分析（STRING）。在分泌组中总共鉴定出239种蛋白质，在外泌体中鉴定出228种特异性蛋白质，共有133种共同蛋白质。分泌组中鉴定出的蛋白质位于细胞外区域和细胞质中，而外泌体蛋白质主要位于膜、细胞质和胞质溶胶中。在功能方面，在分泌组中，参与不同代谢途径、与免疫系统和内分泌系统相关的途径以及内质网中蛋白质加工的蛋白质占主导。相比之下，外泌体特异性蛋白质主要与内吞作用、细胞连接、血小板活化和其他细胞信号通路相关。分泌组或其某些成分（如外泌体）未来可能的应用将为治疗不同疾病提供一种非细胞治疗策略，从而避免细胞治疗的缺点。
