Sung Soo-Eun, Seo Min-Soo, Kang Kyung-Ku, Choi Joo-Hee, Lee Sijoon, Sung Minkyoung, Kim Kilsoo, Lee Gun Woo, Lim Ju-Hyeon, Yang Seung Yun, Yim Sang-Gu, Kim Seul-Ki, Park Sangbum, Kwon Young-Sam, Yun Sungho
Laboratory Animal Center, Daegu-Gyeongbuk Medical Innovation Foundation (DGMIF), Daegu 41061, Korea.
Department of Biomaterials Science (BK21 Four Program), Pusan National University, Miryang 50463, Korea.
Vet Sci. 2021 Sep 3;8(9):182. doi: 10.3390/vetsci8090182.
Adipose tissue-derived mesenchymal stem cells (AD-MSCs) release extracellular vesicles such as exosomes, apoptotic bodies, and microparticles. In particular, exosomes are formed inside cells via multivesicular bodies (MVBs), thus their protein, DNA, and RNA content are similar to those of the parent cells. Exosome research is rapidly expanding, with an increase in the number of related publications observed in recent years; therefore, the function and application of MSC-derived exosomes could emerge as cell-free therapeutics. Exosomes have been isolated from feline AD-MSCs and feline fibroblast cell culture media using ultracentrifugation. Feline exosomes have been characterized by FACS, nanoparticle tracking analysis, and transmission electron microscopy imaging. Moreover, cytokine levels were detected by sandwich enzyme-linked immunosorbent assay in exosomes and LPS-induced THP-1 macrophages. The size of the isolated exosomes was that of a typical exosome, i.e., approximately 150 nm, and they expressed tetraspanins CD9 and CD81. The anti-inflammatory factor IL-10 was increased in feline AD-MSC-derived exosomes. However, pro-inflammatory factors such as IL-1β, IL-8, IL-2, RANTES, and IFN-gamma were significantly decreased in feline AD-MSC-derived exosomes. This was the first demonstration that feline AD-MSC-derived exosomes enhance the inflammatory suppressive effects and have potential for the treatment of immune diseases or as an inflammation-inhibition therapy.
脂肪组织来源的间充质干细胞（AD-MSCs）会释放细胞外囊泡，如外泌体、凋亡小体和微粒。特别是，外泌体在细胞内通过多囊泡体（MVBs）形成，因此其蛋白质、DNA和RNA含量与亲代细胞相似。外泌体研究正在迅速扩展，近年来相关出版物的数量不断增加；因此，间充质干细胞来源的外泌体的功能和应用可能会成为无细胞疗法。已使用超速离心法从猫AD-MSCs和猫成纤维细胞培养基中分离出外泌体。通过流式细胞术、纳米颗粒跟踪分析和透射电子显微镜成像对猫外泌体进行了表征。此外，通过夹心酶联免疫吸附测定法检测了外泌体和脂多糖诱导的THP-1巨噬细胞中的细胞因子水平。分离出的外泌体大小为典型外泌体的大小，即约150nm，并且它们表达四跨膜蛋白CD9和CD81。猫AD-MSC来源的外泌体中抗炎因子IL-10增加。然而，猫AD-MSC来源的外泌体中促炎因子如IL-1β、IL-8、IL-2、RANTES和IFN-γ显著降低。这是首次证明猫AD-MSC来源的外泌体增强炎症抑制作用，并具有治疗免疫疾病或作为炎症抑制疗法的潜力。
