白色念珠菌可溶性细胞质提取物中的细胞壁抗原，通过与伴刀豆球蛋白A的交叉免疫亲和电泳进行鉴定。

Cell wall antigens in soluble cytoplasmic extracts of candida albicans as demonstrated by crossed immuno-affinoelectrophoresis with concanavalin A.

作者信息

Syverson R E, Buckley H R

出版信息

J Immunol Methods. 1977;18(1-2):149-56. doi: 10.1016/0022-1759(77)90166-1.

Abstract

Crossed immuno-affinoelectrophoresis with Concanavalin A precipitated four antigens from soluble extracts of the yeast and mycelial phases of Candida albicans. Two of these antigens were also present in spheroplast preparations. One of the Concanavalin A precipitated antigens was destroyed by periodate and none were digested by pronase. Sequential treatment with pronase and periodate destroyed two of the remaining three antigens. The Concanavalin A precipitated antigens appear to be glycoproteins, two of which are associated with the cell wall of C. albicans.

摘要

伴刀豆球蛋白A交叉免疫亲和电泳从白色念珠菌酵母相和菌丝相的可溶性提取物中沉淀出四种抗原。其中两种抗原也存在于原生质体制备物中。一种伴刀豆球蛋白A沉淀抗原被高碘酸盐破坏，且没有一种被链霉蛋白酶消化。先用链霉蛋白酶处理，再用高碘酸盐处理，破坏了其余三种抗原中的两种。伴刀豆球蛋白A沉淀抗原似乎是糖蛋白，其中两种与白色念珠菌的细胞壁有关。