PICK1 和 BDNF 变异与伊朗人群中甲基苯丙胺依赖风险增加的关联：一项病例对照研究。
Association of PICK1 and BDNF variations with increased risk of methamphetamine dependence among Iranian population: a case-control study.
机构信息
Pharmaceutical Sciences Research Center, Shiraz University of Medical Sciences, Shiraz, Iran.
Department of Medical Genetics and Molecular Medicine, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.
出版信息
BMC Med Genomics. 2021 Jan 26;14(1):27. doi: 10.1186/s12920-021-00873-7.
BACKGROUND
Genetic factors play an important role in susceptibility to methamphetamine dependency. In this line, protein that interact with C-kinase-1 (PICK1) and brain-derived neurotrophic factor (BDNF) genes are linked to methamphetamine dependence (substance use disorder). Thus, in a case-control study, we investigated the association between polymorphisms of PICK1 and BDNF genes and methamphetamine dependence in an Iranian population.
METHODS
Total of 235 cases and 204 controls were recruited in a period between 2015 to 2018. The PICK1-rs713729, -rs2076369 and BDNF-rs6265 genotypes were determined via ARMS-PCR assay. Statistical analysis was performed, using SPSS 20.0, PHASE 2.1.1 program as well as SNP Analyzer 2.0.
RESULTS
In the present study, two polymorphisms including PICK1-rs713729 (OR 1.38 (CI 1.08-1.52; P- 0.004) in multiplicative and dominant models, and PICK1-rs2076369 (OR 1.31 (CI 1.10-1.56; P- 0.002) in multiplicative, dominant and co-dominant models were associated with the risk of methamphetamine abuse. Moreover, haplotype analysis showed a significant association of haplotype AG (OR 2.50 (CI 1.50-4.16; P- 0.0002) in dominant, recessive and co-dominant models, and haplotype TT (OR 0.67 (CI 0.50-0.91; P- 0.009) in dominant and co-dominant models with the risk of methamphetamine abuse. None of the polymorphisms in this study had a high level of linkage disequilibrium.
CONCLUSION
Our findings indicate that the PICK1 gene polymorphism might affect the risk of methamphetamine dependency in our population.
背景
遗传因素在对甲基苯丙胺依赖的易感性中起着重要作用。在这方面，与 C 激酶 1（PICK1）和脑源性神经营养因子（BDNF）基因相互作用的蛋白质与甲基苯丙胺依赖（物质使用障碍）有关。因此，在一项病例对照研究中，我们调查了伊朗人群中 PICK1 和 BDNF 基因多态性与甲基苯丙胺依赖之间的关系。
方法
2015 年至 2018 年间，共招募了 235 例病例和 204 例对照。通过 ARMS-PCR 检测 PICK1-rs713729、-rs2076369 和 BDNF-rs6265 基因型。使用 SPSS 20.0、PHASE 2.1.1 程序和 SNP Analyzer 2.0 进行统计分析。
结果
在本研究中，包括 PICK1-rs713729（OR 1.38（CI 1.08-1.52；P-0.004）在乘法和显性模型中，以及 PICK1-rs2076369（OR 1.31（CI 1.10-1.56；P-0.002）在乘法、显性和共显性模型中，与甲基苯丙胺滥用的风险相关。此外，单体型分析显示单体型 AG（OR 2.50（CI 1.50-4.16；P-0.0002）在显性、隐性和共显性模型中，单体型 TT（OR 0.67（CI 0.50-0.91；P-0.009）在显性和共显性模型中与甲基苯丙胺滥用的风险相关。本研究中没有一个多态性具有高水平的连锁不平衡。
结论
我们的研究结果表明，PICK1 基因多态性可能影响我们人群中甲基苯丙胺依赖的风险。
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