环状RNA_104565通过吸附miR-134促进甲状腺乳头状癌细胞增殖。
CircRNA_104565 Promoted Cell Proliferation in Papillary Thyroid Carcinoma by Sponging miR-134.
作者信息
Gong Jianming, Kong Xiangdong, Qi Jinhui, Lu Jiangkun, Yuan Shaofeng, Wu Ming
机构信息
Department of Surgery, Tinglin Hospital of Jinshan District, Shanghai 201505, People's Republic of China.
出版信息
Int J Gen Med. 2021 Jan 18;14:179-185. doi: 10.2147/IJGM.S288360. eCollection 2021.
BACKGROUND
Thyroid cancer is one of the most common cancers with rising incidence worldwide, and papillary thyroid carcinoma (PTC) accounts for 80-85% of thyroid malignancy. Although it has been reported that many genes relate to the carcinogenesis of PTC, the molecular mechanisms remain mostly unclear.
METHODS
QRT-PCR assay was performed to detect circRNA_104565, miR-134 and ELF2 expression. CCK8 assay was exercised to examine cell proliferation. Western blot was used to detect ELF2 expression.
RESULTS
We found that circRNA_104565 was highly expressed in PTC tissue and cell and promoted cell proliferation in vitro and in vivo. In addition, circRNA_104565 promoted cell proliferation in PTC by regulating the miR-134/ELF2 axis.
CONCLUSION
Hence, revealing the function of circRNA_104565 in PTC is important for understanding the molecular mechanism of carcinogenesis and providing new biomarkers or therapeutic targets for PTC.
背景
甲状腺癌是全球发病率不断上升的最常见癌症之一，甲状腺乳头状癌（PTC）占甲状腺恶性肿瘤的80 - 85%。尽管已有报道称许多基因与PTC的致癌作用有关，但其分子机制大多仍不清楚。
方法
进行QRT - PCR检测以检测circRNA_104565、miR - 134和ELF2的表达。采用CCK8检测法检测细胞增殖。使用蛋白质免疫印迹法检测ELF2的表达。
结果
我们发现circRNA_104565在PTC组织和细胞中高表达，并在体外和体内促进细胞增殖。此外，circRNA_104565通过调节miR - 134/ELF2轴促进PTC中的细胞增殖。
结论
因此，揭示circRNA_104565在PTC中的功能对于理解致癌分子机制以及为PTC提供新的生物标志物或治疗靶点具有重要意义。
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