Hassan Basima, Omran Mohamed M, Youssef Magdy M, Refaat Manar, Abouzid Amr, Abdel Wahab Abdel Hady A, Bakr Yasser M
Biochemistry Division, Chemistry Department, Faculty of Science, Mansoura University, Mansoura, Egypt.
Chemistry Department, Faculty of Science, Helwan University, Cairo, Egypt.
Technol Cancer Res Treat. 2024 Jan-Dec;23:15330338241291637. doi: 10.1177/15330338241291637.
Thyroid cancer, a prevalent endocrine malignancy, often presents as thyroid nodules, whose benign or malignant nature is challenging to determine. This study aims to identify circulating miRNA panels that may distinguish between benign nodules, papillary thyroid cancer, and normal thyroid conditions, building on extensive research into miRNAs as potential thyroid cancer biomarkers.
As a cross-sectional case-control study the study revealed the quantification of the 17-miRNA panel was evaluated using qRT-PCR method on 60 blood samples, comprising 25 patients diagnosed with PTC, 24 patients with benign lesions, and 11 healthy controls.
We found that seven miRNAs, including miR144-3p and miR190a-5p, exhibited statistically significant variations in their expression levels, and miR144-3p exhibited the most superior diagnostic capability in distinguishing between malignant and healthy groups, as well as between benign and healthy groups. The concurrent utilization of miR144-3p, miR148a-3p, and miR190a-5p resulted in an enhancement of sensitivity from 76% to 88%.
Our study suggests that miR144-3p and miR190a-5p exhibit potential as biomarkers for distinguishing between benign and malignant thyroid nodules and healthy individuals, and further investigation is necessary to evaluate their clinical significance.
甲状腺癌是一种常见的内分泌恶性肿瘤，常表现为甲状腺结节，其良恶性难以确定。本研究旨在基于对作为潜在甲状腺癌生物标志物的微小RNA（miRNA）的广泛研究，确定可区分良性结节、甲状腺乳头状癌和正常甲状腺状态的循环miRNA组合。
作为一项横断面病例对照研究，该研究使用qRT-PCR方法对60份血样中的17-miRNA组合进行定量评估，这些血样包括25例诊断为甲状腺乳头状癌（PTC）的患者、24例良性病变患者和11例健康对照。
我们发现包括miR144-3p和miR190a-5p在内的7种miRNA在表达水平上表现出统计学上的显著差异，且miR144-3p在区分恶性与健康组以及良性与健康组方面表现出最优异的诊断能力。同时使用miR144-3p、miR148a-3p和miR190a-5p可使灵敏度从76%提高到88%。
我们的研究表明，miR144-3p和miR190a-5p具有作为区分良性和恶性甲状腺结节以及健康个体的生物标志物的潜力，有必要进一步研究以评估它们的临床意义。
