Zemplenyi T, Crawford D W, Tidwell D
University of Southern California, School of Medicine, Los Angeles 90033.
Exp Mol Pathol. 1988 Apr;48(2):252-61. doi: 10.1016/0014-4800(88)90062-7.
In eight New Zealand white male rabbits the abdominal aorta and one iliofemoral artery was balloon deendothelialized (group A). After 2 weeks they were kept for 6 weeks on a high cholesterol diet together with eight unoperated rabbits (group B). Eight more rabbits were kept on a commercial diet only (group C). The degree of atherosclerosis was much higher in the deendothelialized Group A vessels than in the uninjured group B vessels. The activity of lactate dehydrogenase and of the rate-limiting glycolytic pyruvate kinase was significantly increased and the activity of lipoamide dehydrogenase decreased in the group A aortas. In the iliofemoral arteries a similar but statistically insignificant tendency was detected. There was no significant difference, however, in aortic lactate between the three groups. Thus, local hypoxia did not significantly contribute to the high degree of atherosclerosis in the group A animals in spite of the enzyme activity differences. Previous experience of the authors, using arterial microcathode pO2 measurements, indicates that following deendothelialization an adaptive proliferation of nutrient vessels and increased arterial oxygenation takes place. The average activity of the lysosomal N-acetyl-beta-glucosaminidase was five times and that of beta-glucuronidase, seven times higher in the Group A than Group B aortas; in the iliofemoral arteries the differences were even larger. The huge elevation of these hydrolases, which are involved in glycosaminoglycan catabolism, provides indirect indication that accumulation of glycosaminoglycans and possibly their ability to form complexes with apoB-containing lipoproteins played a major role in the much increased degree of atherosclerotic lesions in the Group A rabbits.
在八只新西兰雄性白兔中，对腹主动脉和一条髂股动脉进行球囊去内皮处理（A组）。两周后，它们与八只未做手术的兔子一起接受六周的高胆固醇饮食（B组）。另外八只兔子仅给予普通饮食（C组）。去内皮的A组血管中动脉粥样硬化程度比未受伤的B组血管高得多。A组主动脉中乳酸脱氢酶和限速糖酵解丙酮酸激酶的活性显著增加，而硫辛酰胺脱氢酶的活性降低。在髂股动脉中检测到类似但无统计学意义的趋势。然而，三组之间主动脉乳酸水平无显著差异。因此，尽管酶活性存在差异，但局部缺氧对A组动物高度的动脉粥样硬化没有显著影响。作者先前使用动脉微阴极pO2测量的经验表明，去内皮后会发生营养血管的适应性增殖和动脉氧合增加。A组主动脉中溶酶体N-乙酰-β-氨基葡萄糖苷酶的平均活性是B组的五倍，β-葡萄糖醛酸酶的平均活性是B组的七倍；在髂股动脉中差异更大。这些参与糖胺聚糖分解代谢的水解酶大幅升高，间接表明糖胺聚糖的积累及其与含载脂蛋白B的脂蛋白形成复合物的能力在A组兔子动脉粥样硬化病变程度大幅增加中起主要作用。
