P. E. Department of Jiangsu University, Zhenjiang, China.
School of Automation, Hangzhou Dianzi University, Hangzhou, China.
Luminescence. 2021 Jun;36(4):842-848. doi: 10.1002/bio.4017. Epub 2021 Feb 7.
Rolling circle amplification (RCA) is an isothermal process under the action of DNA polymerases. Large-scale DNA templates have been generated using RCA for target detection. Some signal amplification strategies including optical sensors and electrochemical sensors based on RCA have been applied to achieve sensitive detection. Sensors based on RCA have attracted increasing interest. Advances in RCA-based sensors for protein detection are reviewed in this paper. The advantages and detection mechanisms of sensors based on RCA are revealed and discussed. Finally, possible challenges and future perspectives are also outlined.
滚环扩增（RCA）是在 DNA 聚合酶作用下的等温过程。已经使用 RCA 生成了用于目标检测的大规模 DNA 模板。一些信号放大策略，包括基于 RCA 的光学传感器和电化学传感器，已被应用于实现灵敏检测。基于 RCA 的传感器引起了越来越多的关注。本文综述了基于 RCA 的蛋白质检测传感器的进展。揭示并讨论了基于 RCA 的传感器的优点和检测机制。最后，还概述了可能的挑战和未来展望。
