College of Chemistry and Chemical Engineering, Qingdao University, Qingdao 266071, PR China.
Laboratory of Optic-Electric Sensing and Analytical Chemistry for Life Science, Ministry of Education, Shandong Key Laboratory of Biochemical Analysis, Key Laboratory of Analytical Chemistry for Life Science in Universities of Shandong, College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao 266042, PR China.
Trends Biotechnol. 2021 Nov;39(11):1160-1172. doi: 10.1016/j.tibtech.2021.02.007. Epub 2021 Mar 11.
Rolling circle replication (RCR), including rolling circle amplification (RCA) and rolling circle transcription (RCT), is an isothermal enzymatic reaction. Because of its high amplification efficiency, RCR is a powerful biosensing tool for detecting biomolecules. In recent years, RCR has also been extended to the field of bioimaging to better understand biological pathways. Furthermore, RCR provides a simple technique to design and generate DNA/RNA structures with unique advantages in delivering drugs and enhanced targeting ability. In this review, we introduce the fundamentals of RCR and describe the most recent advances in RCR-based detection methods and delivery vehicles for biosensing, bioimaging, and biomedicine. Finally, some challenges and further opportunities of RCR-based biotechnology are discussed.
滚环复制（RCR），包括滚环扩增（RCA）和滚环转录（RCT），是一种等温酶反应。由于其高扩增效率，RCR 是一种用于检测生物分子的强大生物传感工具。近年来，RCR 也已扩展到生物成像领域，以更好地了解生物途径。此外，RCR 提供了一种简单的技术来设计和生成具有独特优势的 DNA/RNA 结构，在药物输送和增强靶向能力方面具有优势。在这篇综述中，我们介绍了 RCR 的基本原理，并描述了基于 RCR 的检测方法和生物传感、生物成像和生物医学的输送载体的最新进展。最后，讨论了基于 RCR 的生物技术的一些挑战和进一步的机会。
