Yan Feifei, Lv Minchao, Zhang Tie, Zhang Qi, Chen Yan, Liu Zhibo, Wei Renxiong, Cai Lin
Department of Orthopedics, Zhongnan Hospital of Wuhan University, No. 169, Donghu Road, Wuhan 430071, China.
Hubei Osteolink Biomaterial Co., Ltd. (Wuhan Hi-tech Research Center of Medical Tissues), No. 379, Gaoxiner Road, Wuhan 430100, China.
ACS Biomater Sci Eng. 2021 Feb 8;7(2):663-671. doi: 10.1021/acsbiomaterials.0c01471. Epub 2021 Jan 27.
Traditional bone wax has lots of shortcomings such as the risk of infection and inflammation and the ability to hinder osteogenesis that limit its clinical applications. In this study, we designed a novel biodegradable bone wax with desirable angiogenic and antibacterial ability and low foreign body reaction by mixing calcium sulfate, poloxamer, and cupric ions. To evaluate its biocompatibility and angiogenetic effect , we cultured human umbilical vein endothelial cells (HUVECs) with the indicated bone wax to observe cell viability and vessel-like tubular formation. The bone wax was then implanted in a critical-sized bone defect rat model for 4 and 8 weeks to successfully stimulate angiogenesis . Finally, the bone wax extract was incubated with Gram-positive to confirm its antibacterial ability. The copper-loaded biodegradable bone wax overcomes the drawbacks of traditional bone wax and provides a new approach for the treatment of bone injuries.
传统骨蜡存在诸多缺点，如感染和炎症风险以及阻碍骨生成的能力，这些都限制了其临床应用。在本研究中，我们通过将硫酸钙、泊洛沙姆和铜离子混合，设计了一种具有理想血管生成和抗菌能力且异物反应低的新型可生物降解骨蜡。为评估其生物相容性和血管生成作用，我们用指定的骨蜡培养人脐静脉内皮细胞（HUVECs），以观察细胞活力和血管样管状结构的形成。然后将骨蜡植入临界尺寸骨缺损大鼠模型中4周和8周，以成功刺激血管生成。最后，将骨蜡提取物与革兰氏阳性菌一起孵育，以确认其抗菌能力。载铜可生物降解骨蜡克服了传统骨蜡的缺点，为骨损伤治疗提供了一种新方法。
