开发一种基于单克隆抗体的免疫层析试纸条，用于快速检测人血清中的替加环素。

Development of a monoclonal antibody-based immunochromatographic strip for the rapid detection of tigecycline in human serum.

作者信息

Lu Qianqian, Xu Xinxin, Song Shanshan, Wu Aihong, Liu Liqiang, Kuang Hua, Xu Chuanlai

机构信息

State Key Lab of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu 214122, People's Republic of China.

出版信息

Anal Methods. 2021 Feb 19;13(6):817-824. doi: 10.1039/d0ay02182b.

DOI:10.1039/d0ay02182b

PMID:33502396

Abstract

In this study, a gold labelled immunochromatographic assay was developed to detect tigecycline (TGC) in human serum. For this purpose, an anti-TGC monoclonal antibody, 2G7, was produced and characterized, and was found to have a 50%-inhibitory concentration (IC50) of 2.303 ng mL-1 and a limit of detection (LOD) of 0.215 ng mL-1 for TGC. This strip assay had a visual limit of detection (vLOD) of 50 ng mL-1 and a cut-off value of 1000 ng mL-1 for TGC in human serum, when assessed by eye. With the aid of a strip scan reader, a quantitative determination of TGC was obtained with a calculated limit of detection (cLOD) of 15.03 ng mL-1. Analysis of TGC in human serum indicated that the results of our strip assay compared well with results obtained using ic-ELISA and LC-MS/MS. Therefore, this strip assay represents a sensitive and reliable method for the on-site detection of TGC in serum samples.

摘要

在本研究中，开发了一种金标免疫层析法用于检测人血清中的替加环素（TGC）。为此，制备并表征了一种抗TGC单克隆抗体2G7，发现其对TGC的50%抑制浓度（IC50）为2.303 ng/mL，检测限（LOD）为0.215 ng/mL。当通过肉眼评估时，该条带分析法对人血清中TGC的视觉检测限（vLOD）为50 ng/mL，临界值为1000 ng/mL。借助条带扫描读数器，获得了TGC的定量测定结果，计算检测限（cLOD）为15.03 ng/mL。人血清中TGC的分析表明，我们的条带分析法结果与免疫竞争酶联免疫吸附测定法（ic-ELISA）和液相色谱-串联质谱法（LC-MS/MS）获得的结果相当。因此，该条带分析法是一种用于血清样品中TGC现场检测的灵敏且可靠的方法。