采用 ELISA 和基于金纳米粒子的免疫层析试验条同时检测非那西汀和对乙酰氨基酚。
Simultaneous detection of phenacetin and paracetamol using ELISA and a gold nanoparticle-based immunochromatographic test strip.
机构信息
State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, People's Republic of China.
International Joint Research Laboratory for Biointerface and Biodetection, and School of Food Science and Technology, Jiangnan University, Wuxi, People's Republic of China.
出版信息
Analyst. 2021 Oct 11;146(20):6228-6238. doi: 10.1039/d1an01173a.
We have developed a sensitive and rapid gold nanoparticle-based immunochromatographic strip (GNP-ICS) for the detection of phenacetin (PNCT) and paracetamol (PAP) using an anti-PNCT monoclonal antibody (mAb). The sensitive anti-PNCT mAb (2D6) had a half maximal inhibitory concentration (IC) and limit of detection (LOD) of 3.51 and 0.21 ng mL, respectively. Additionally, its cross-reactivity with PAP was approximately 10.1%. The developed GNP-ICS assay based on GNP-labeled mAb was sensitive for the detection of PNCT with vLOD and cut-off values of 2.5 and 50 ng mL respectively and a vLOD value of 25 ng mL for PAP. Furthermore, the developed ELISA and GNP-ICS assays were applied to determine PNCT-spiked beverage samples without pretreatment, in addition to a kind of PAP-containing drug. The recoveries were validated using high performance liquid chromatography (HPLC). The results revealed that the developed GNP-ICS assay was reliable for the detection of PNCT in practical samples.
我们开发了一种基于金纳米颗粒的免疫层析条（GNP-ICS），用于使用抗非那西汀单克隆抗体（mAb）检测非那西汀（PNCT）和扑热息痛（PAP）。灵敏的抗非那西汀 mAb（2D6）的半抑制浓度（IC）和检测限（LOD）分别为 3.51 和 0.21ng/mL。此外，它与扑热息痛的交叉反应性约为 10.1%。基于 GNP 标记 mAb 的开发的 GNP-ICS 测定法对 PNCT 的检测具有低检测限（vLOD）和截止值分别为 2.5 和 50ng/mL，以及 PAP 的 vLOD 值为 25ng/mL。此外，还使用高效液相色谱法（HPLC）验证了开发的 ELISA 和 GNP-ICS 测定法来确定未经预处理的 PNCT 加标饮料样品，以及一种含有 PAP 的药物。回收验证使用高效液相色谱法（HPLC）进行。结果表明，开发的 GNP-ICS 测定法可用于检测实际样品中的 PNCT。
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