State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, People's Republic of China; International Joint Research Laboratory for Biointerface and Biodetection, School of Food Science and Technology, Jiangnan University, Wuxi, People's Republic of China.
State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, People's Republic of China; International Joint Research Laboratory for Biointerface and Biodetection, School of Food Science and Technology, Jiangnan University, Wuxi, People's Republic of China.
Food Chem. 2021 Sep 15;356:129710. doi: 10.1016/j.foodchem.2021.129710. Epub 2021 Mar 27.
We developed a sensitive and rapid lateral flow immunochromatographic (LFI) assay for the simultaneous detection of fipronil and its metabolites in eggs and cucumbers using gold nanoparticle (GNP)-labeled monoclonal antibodies (mAbs). Anti-fipronil mAbs (1B6) were produced using two haptens and identified by heterologous indirect competitive enzyme-linked immunosorbent assay (icELISA) with half maximal inhibitory concentration (IC) and limit of detection (LOD) values of 0.46 ± 0.07 and 0.05 ± 0.01 ng mL, respectively. The developed LFI strip showed high sensitivity and specificity in the detection of fipronil with cut-off and visual limit of detection (vLOD) values of 10 and 0.25 ng mL, respectively. Furthermore, the application of LFI in the detection of fipronil-spiked egg and cucumber samples was validated by liquid chromatography tandem mass spectrometry (LC-MS/MS). Our developed LFI assay is suitable for detection of fipronil and its metabolites in real samples.
我们开发了一种灵敏、快速的侧向流动免疫层析（LFI）检测方法，用于同时检测鸡蛋和黄瓜中的氟虫腈及其代谢物，该方法使用金纳米颗粒（GNP）标记的单克隆抗体（mAb）。使用两种半抗原制备了抗氟虫腈 mAb（1B6），并通过异源间接竞争酶联免疫吸附测定（icELISA）进行了鉴定，半数抑制浓度（IC）和检测限（LOD）值分别为 0.46 ± 0.07 和 0.05 ± 0.01 ng/mL。开发的 LFI 条带在检测氟虫腈时具有高灵敏度和特异性，截断值和视觉检测限（vLOD）值分别为 10 和 0.25 ng/mL。此外，通过液相色谱串联质谱（LC-MS/MS）验证了 LFI 在检测添加氟虫腈的鸡蛋和黄瓜样品中的应用。我们开发的 LFI 检测方法适用于检测真实样品中的氟虫腈及其代谢物。
