Department of Neurosurgery, Cangzhou Central Hospital, Cangzhou, Hebei, People's Republic of China.
PLoS One. 2021 Jan 27;16(1):e0246097. doi: 10.1371/journal.pone.0246097. eCollection 2021.
Low-grade gliomas (LGGs) is a primary invasive brain tumor that grows slowly but is incurable and eventually develops into high malignant glioma. Novel biomarkers for the tumorigenesis and lifetime of LGG are critically demanded to be investigated. In this study, the expression levels of procollagen-lysine, 2-oxoglutarate 5-dioxygenases (PLODs) were analyzed by ONCOMINE, HPA and GEPIA. The GEPIA online platform was applied to evaluate the interrelation between PLODs and survival index in LGG. Furthermore, functions of PLODs and co-expression genes were inspected by the DAVID. Moreover, we used TIMER, cBioportal, GeneMINIA and NetworkAnalyst analysis to reveal the mechanism of PLODs in LGG. We found that expression levels of each PLOD family members were up-regulated in patients with LGG. Higher expression of PLODs was closely related to shorter disease-free survival (DFS) and overall survival (OS). The findings showed that LGG cases with or without alterations were significantly correlated with the OS and DFS. The mechanism of PLODs in LGG may be involved in response to hypoxia, oxidoreductase activity, Lysine degradation and immune cell infiltration. In general, this research has investigated the values of PLODs in LGG, which could serve as biomarkers for diagnosis, prognosis and potential therapeutic targets of LGG patients.
低级别胶质瘤（LGG）是一种原发性侵袭性脑肿瘤，生长缓慢，但无法治愈，最终会发展成高度恶性胶质瘤。因此，迫切需要研究用于肿瘤发生和 LGG 患者生存期的新型生物标志物。在这项研究中，通过 ONCOMINE、HPA 和 GEPIA 分析了前胶原赖氨酸、2-氧戊二酸 5-双加氧酶（PLOD）的表达水平。应用 GEPIA 在线平台评估了 PLOD 与 LGG 生存指数之间的关系。此外，通过 DAVID 检查了 PLOD 的功能和共表达基因。还使用了 TIMER、cBioportal、GeneMINIA 和 NetworkAnalyst 分析来揭示 PLOD 在 LGG 中的作用机制。结果发现，每个 PLOD 家族成员的表达水平在 LGG 患者中均上调。PLOD 的高表达与无病生存期（DFS）和总生存期（OS）较短密切相关。结果表明，有无改变的 LGG 病例与 OS 和 DFS 显著相关。PLOD 在 LGG 中的作用机制可能涉及对缺氧、氧化还原酶活性、赖氨酸降解和免疫细胞浸润的反应。总的来说，这项研究调查了 PLOD 在 LGG 中的价值，它可以作为 LGG 患者诊断、预后和潜在治疗靶点的生物标志物。
