利用环保提取技术提高废咖啡渣中酚类化合物的回收率。

Improving the Recovery of Phenolic Compounds from Spent Coffee Grounds (SCG) by Environmentally Friendly Extraction Techniques.

机构信息

Department of Food Engineering, Middle East Technical University, 06800 Ankara, Turkey.

Department of Food Engineering, Niğde Ömer Halisdemir University, 51240 Niğde, Turkey.

出版信息

Molecules. 2021 Jan 25;26(3):613. doi: 10.3390/molecules26030613.

DOI:10.3390/molecules26030613

PMID:33503910

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7865687/

Abstract

The aim of this study was to investigate and compare the effects of different extraction techniques (high hydrostatic pressure-assisted extraction (HHPE), ultrasound-assisted extraction (UAE), and classical solvent extraction (CSE)) on phenolic compounds from spent coffee grounds (SCG). Different HHPE parameters (300, 400 and 500 MPa at 25 °C for 5, 10 and 15 min) and UAE parameters (40%, 50%, and 60% amplitude at 25 °C for 5, 10 and 15 min) were used. These techniques were compared with CSE (at 50 °C for 30 min) according to total phenolic content (TPC), antioxidant activity (AA), high-performance liquid chromatography (HPLC), scanning electron microscopy (SEM), and infrared (IR) spectroscopy. The results showed that eco-friendly techniques increased the TPC and AA compared to CSE and morphological changes were verified by SEM results. Furthermore, chlorogenic and caffeic acid were also quantified by using HPLC. Chlorogenic acid was found as the main phenolic compound in spent coffee grounds (SCG). The highest chlorogenic acid was detected as 85.0 ± 0.6 mg/kg FW with UAE at 60% amplitude for 15 min. In brief, for the extraction of phenolic compounds from waste SCG eco-friendly techniques such as HHPE and/or UAE were more convenient than CSE.

摘要

本研究旨在探讨和比较不同提取技术（高静压辅助提取（HHPE）、超声辅助提取（UAE）和经典溶剂提取（CSE））对废咖啡渣（SCG）中酚类化合物的影响。使用了不同的 HHPE 参数（25°C 下 300、400 和 500 MPa 分别为 5、10 和 15 分钟）和 UAE 参数（25°C 下 40%、50%和 60%的振幅分别为 5、10 和 15 分钟）。这些技术根据总酚含量（TPC）、抗氧化活性（AA）、高效液相色谱（HPLC）、扫描电子显微镜（SEM）和红外（IR）光谱与 CSE（50°C 下 30 分钟）进行了比较。结果表明，与 CSE 相比，环保技术提高了 TPC 和 AA，并且 SEM 结果验证了形态变化。此外，还使用 HPLC 定量测定了绿原酸和咖啡酸。在废咖啡渣（SCG）中发现绿原酸是主要的酚类化合物。用 UAE 在 60%振幅下提取 15 分钟，检测到的绿原酸最高为 85.0±0.6mg/kg FW。总之，对于从废 SCG 中提取酚类化合物，环保技术如 HHPE 和/或 UAE 比 CSE 更方便。