Chopjitt Peechanika, Putthanachote Nuntiput, Ungcharoen Ratchadaporn, Hatrongjit Rujirat, Boueroy Parichart, Akeda Yukihiro, Tomono Kazunori, Hamada Shigeyuki, Kerdsin Anusak
Faculty of Public Health, Chalermphrakiat Sakon Nakhon Campus, Kasetsart University, Sakon Nakhon 47000, Thailand.
Clinical Microbiology Laboratory, Roi-Et Hospital, Roi-Et 45000, Thailand.
Microorganisms. 2021 Jan 25;9(2):242. doi: 10.3390/microorganisms9020242.
Carbapenem-resistant (CRAP) is a causative agent of nosocomial infections. This study aimed to characterize clinical isolates of CRAP from a tertiary hospital in Northeast Thailand. Six isolates were confirmed as extensively drug-resistant (XDRAP). The gene was detected in three isolates, whereas and were detected in the others. Multilocus sequence typing with the Pasteur scheme revealed ST220 in two isolates, ST744 in two isolates, and ST63 and ST396 for the remaining two isolates, respectively. Genomic characterization revealed that six XDRAP genes contained antimicrobial resistance genes: ST63 (A436) and ST396 (A1) contained 10 antimicrobial resistance genes, ST220 (A984 and A864) and ST744 (A56 and A273) contained 9 and 8 antimicrobial resistance genes, respectively. The single nucleotide polymorphism (SNP) phylogenetic tree revealed that the isolates A984 and A864 were closely related to YB-45 (ST220) from China, while A436 was related to WCHAP100020, also from China. A273 and A56 isolates (ST744) were clustered together; these isolates were closely related to strains 2014S07-126, AP43, and WCHAP005069, which were isolated from Taiwan and China. Strict implementation of infection control based upon the framework of epidemiological analyses is essential to prevent outbreaks and contain the spread of the pathogen. Continued surveillance and close monitoring with molecular epidemiological tools are needed.
耐碳青霉烯类（CRAP）是医院感染的病原体。本研究旨在对泰国东北部一家三级医院的CRAP临床分离株进行特征分析。六株分离株被确认为广泛耐药（XDRAP）。在三株分离株中检测到了该基因，而在其他分离株中检测到了 和 。采用巴斯德方案进行多位点序列分型，结果显示两株分离株为ST220，两株为ST744，其余两株分别为ST63和ST396。基因组特征分析表明，六个XDRAP基因含有抗菌抗性基因：ST63（A436）和ST396（A1）含有10个抗菌抗性基因，ST220（A984和A864）和ST744（A56和A273）分别含有9个和8个抗菌抗性基因。单核苷酸多态性（SNP）系统发育树显示，分离株A984和A864与来自中国的 YB - 45（ST220）密切相关，而A436与同样来自中国的WCHAP100020相关。A273和A56分离株（ST744）聚在一起；这些分离株与从台湾和中国分离出的菌株2014S07 - 126、AP43和WCHAP005069密切相关。基于流行病学分析框架严格实施感染控制对于预防疫情爆发和遏制病原体传播至关重要。需要持续进行监测并使用分子流行病学工具进行密切监测。
