Wang Shengke, Zhou Yan, Wang Yuezhuo, Tang Keshu, Wang Danqi, Hong Jiawen, Wang Pengcheng, Ye Sheng, Yan Jie, Li Shengkai, Zhou Zhemin, Du Jimei
Wenzhou Key Laboratory of Sanitary Microbiology, Department of Microbiology and Immunology, School of Laboratory Medicine, Institute of One Health, Wenzhou Medical University, Wenzhou, China.
School of Medicine, Zhejiang University, Hangzhou, China.
Commun Biol. 2025 May 13;8(1):738. doi: 10.1038/s42003-025-08156-y.
As a member of Acinetobacter calcoaceticus-baumannii complex, Acinetobacter pittii has been an emerging concern in nosocomial infection due to its increasing prevalence and multidrug resistance (MDR). However, its population structure remains broadly unknown, hampering efficient tracing of its transmission and evolution. In this study, we developed a distributed core genome multilocus sequence typing (dcgMLST) for A. pittii based on 750 genomes and employed it to map the genetic landscape and evolution of A. pittii. The results demonstrated that two hierarchical clustering (HC) levels effectively correspond to genetic diversity from species (HC1100) to natural populations (HC450), as well as that a predominant lineage, HC1100_4, accounts for 33.9% of A. pittii strains. Subsequent analysis revealed that specific gene gain and loss events within HC1100_4 are linked to adaptations to environmental stress. Moreover, we identified a cluster of multidrug-resistant plasmids PT_712 responsible for the dissemination of bla genes within the genus of Acinetobacter. This study provides a framework for characterizing genetic diversity, evolutionary dynamics, molecular population distribution, and tracing of A. pittii, which has the potential to improve infection control strategies and public health policy.
作为醋酸钙不动杆菌-鲍曼不动杆菌复合体的一员，皮氏不动杆菌因其日益增加的流行率和多重耐药性（MDR），已成为医院感染中一个新出现的问题。然而，其种群结构仍大致未知，这阻碍了对其传播和进化的有效追踪。在本研究中，我们基于750个基因组开发了一种用于皮氏不动杆菌的分布式核心基因组多位点序列分型（dcgMLST），并利用它来描绘皮氏不动杆菌的遗传图谱和进化情况。结果表明，两个层次聚类（HC）水平有效地对应了从物种（HC1100）到自然种群（HC450）的遗传多样性，并且一个主要谱系HC1100_4占皮氏不动杆菌菌株的33.9%。随后的分析表明，HC1100_4内特定的基因获得和丢失事件与对环境压力的适应有关。此外，我们鉴定出一组负责鲍曼不动杆菌属内bla基因传播的多重耐药质粒PT_712。本研究为表征皮氏不动杆菌的遗传多样性、进化动态、分子种群分布和追踪提供了一个框架，这有可能改善感染控制策略和公共卫生政策。
