Scotti Raffaella, Stringaro Annarita, Nicolini Laura, Zanellato Miriam, Boccia Priscilla, Maggi Filippo, Gabbianelli Roberta
Biological Service, Italian National Institute of Health, 00161 Rome, Italy.
National Center for Drug Research and Evaluation, Italian National Institute of Health, 00161 Rome, Italy.
Antibiotics (Basel). 2021 Jan 25;10(2):113. doi: 10.3390/antibiotics10020113.
Every year, the pharmaceutical and food industries produce over 1000 tons of essential oils (EOs) exploitable in different fields as the development of eco-friendly and safe antimicrobial inhibitors. In this work we investigated the potential of some EOs, namely , , and , on the growth, biofilm formation and gene expression in four strains of enterohemorrhagic O157:H7. All EOs were analyzed by gas chromatography-mass spectrometry (GC-MS). The antimicrobial activity was performed by using dilutions of EOs ranging from 0.001 to 1.2% (v/v). Subinhibitory doses were used for biofilm inhibition assay. The expression profiles were obtained by RT-PCR. O157:H7 virulence was evaluated in vivo in the nematode . All EOs showed minimal inhibitory concentrations (MICs) ranging from 0.0075 to 0.3% (v/v). bark EO had the best activity (MIC of 0.0075% (v/v) in all strains) while the leaf EO had an intermediate efficacy with MIC of 0.175% (v/v) in almost all strains. The spp. showed the more variable MICs (ranging from 0.075 to 0.3% (v/v)) depending on the strain used. Transcriptional analysis showed that EO repressed several genes involved in biofilm formation, virulence, zinc homeostasis and encoding some membrane proteins. All EOs affected zinc homeostasis, reducing M and T expression, and reduced the ability of O157:H7 to infect the nematode . In conclusion, we demonstrated that these EOs, affecting O157:H7 infectivity, have a great potential to be used against infections caused by microorganisms.
每年，制药和食品行业都会生产超过1000吨的香精油（EOs），这些香精油可作为环保且安全的抗菌抑制剂应用于不同领域。在这项研究中，我们调查了某些香精油，即[具体名称1]、[具体名称2]、[具体名称3]和[具体名称4]，对四株肠出血性大肠杆菌O157:H7的生长、生物膜形成和基因表达的影响。所有香精油均通过气相色谱 - 质谱联用仪（GC - MS）进行分析。通过使用浓度范围为0.001%至1.2%（v/v）的香精油稀释液来测定抗菌活性。亚抑菌剂量用于生物膜抑制试验。通过逆转录 - 聚合酶链反应（RT - PCR）获得表达谱。在秀丽隐杆线虫体内评估O157:H7的毒力。所有香精油的最低抑菌浓度（MICs）范围为0.0075%至0.3%（v/v）。[具体名称1]树皮香精油活性最佳（在所有菌株中的MIC为0.0075%（v/v）），而[具体名称2]叶香精油具有中等效力，在几乎所有菌株中的MIC为0.175%（v/v）。[具体名称3]属根据所使用的菌株显示出更具变化性的MICs（范围为0.075%至0.3%（v/v））。转录分析表明，[具体名称1]香精油抑制了多个参与生物膜形成、毒力、锌稳态以及编码某些膜蛋白的基因。所有香精油均影响锌稳态，降低M和T的表达，并降低O157:H7感染线虫的能力。总之，我们证明这些香精油通过影响O157:H7的感染性，具有用于对抗微生物感染的巨大潜力。
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