Gomathinayagam Sankaranarayanan, Kodiveri Muthukaliannan Gothandam
School of Biosciences and Technology, Vellore Institute of Technology, Vellore, Tamilnadu 632014 India.
Indian J Microbiol. 2021 Mar;61(1):38-44. doi: 10.1007/s12088-020-00909-z. Epub 2020 Oct 7.
Rapidly evolving sequencing technologies have enabled efficient sequencing of complex genomes and metagenomes. Here, we have presented our metagenomic analysis of rat faeces isolated DNA, sequenced using long-read sequencing technology. The microbiome changes in the rat faeces after sixteen weeks of prolonged administration of subcutaneous 1,2 dimethylhydrazine to induce colon carcinogenesis and oral carotenoid-rich whole-cell lyophilised supplement. The faecal pellets were aseptically collected, and DNA was isolated and sequenced subsequently. The post-sequencing analysis revealed that the rat gut microbiome is highly complex and diverse. There was a significant difference between the microbiome of rats that received supplement in comparison with rats treated with 1,2 dimethylhydrazine and control rats. We observed the dominance of over in both cases of administration. The dominance was notably contributed by individuals like , and The presence of protozoans like , and was also identified among other commensal eukaryotes. Moreover, there was an abundant presence of bacteriophages targeting probiotic organisms like among the identified DNA viruses.
快速发展的测序技术已能够对复杂基因组和宏基因组进行高效测序。在此，我们展示了对大鼠粪便分离DNA的宏基因组分析，该DNA使用长读长测序技术进行测序。在皮下注射1,2 - 二甲基肼诱导结肠癌发生并口服富含类胡萝卜素的全细胞冻干补充剂十六周后，大鼠粪便中的微生物组发生了变化。粪便颗粒经无菌收集，随后分离DNA并进行测序。测序后分析表明，大鼠肠道微生物组高度复杂且多样。与接受1,2 - 二甲基肼处理的大鼠和对照大鼠相比，接受补充剂的大鼠的微生物组存在显著差异。在两种给药情况下，我们都观察到[具体微生物名称1]相对于[具体微生物名称2]占优势。这种优势尤其由[具体微生物名称3]、[具体微生物名称4]等个体促成。在其他共生真核生物中也鉴定出了[具体原生动物名称1]、[具体原生动物名称2]等原生动物的存在。此外，在已鉴定的DNA病毒中，存在大量靶向益生菌如[具体益生菌名称]的噬菌体。
