School of Chinese Materia Medica, Beijing University of Chinese Medicine, No.11 North 3rd Ring East Road, Chao-Yang District, Beijing, 100029, China.
School of Life Sciences, Beijing University of Chinese Medicine, Intersection of Yang-Guang South Street and Bai-Yang East Road, Fang-Shan District, Beijing, 102488, China.
Biomed Pharmacother. 2020 Apr;124:109873. doi: 10.1016/j.biopha.2020.109873. Epub 2020 Jan 24.
The incidence of type 1 diabetes mellitus (T1DM) is increasing year by year, gut microbiota is considered to be closely related to the occurrence and development of T1DM in recent years. In this study, Sprague Dawley (SD) rats were intraperitoneally injected with 75mg/kg streptozotocin to establish T1DM model, fecal samples were collected and DNA were extracted, 16S rRNA microbial gene clone library were constructed, and lastly high-throughput sequencing and bioinformatics analysis were performed. The results showed that the abundances of pathogenic bacteria such as Ruminococcaceae, Shigella, Enterococcus, Streptococcus, Rothia and Alistipes associated with infection and inflammation in T1DM rats were up-regulated, while the abundances of beneficial bacteria such as Lactobacillus, Faecalitalea, Butyricicoccus and Allobaculum were reduced. Among them, Butyricicoccus and Allobaculum protect intestinal barrier function by producing short-chain fatty acids. This study suggests that intestinal inflammation and reduction of short chain fatty acids (SCFAs) caused by the imbalance of gut microbiota are crucial to the pathogenesis of T1DM.
1 型糖尿病（T1DM）的发病率逐年上升，近年来，肠道微生物群被认为与 T1DM 的发生和发展密切相关。本研究采用腹腔注射链脲佐菌素（75mg/kg）建立 T1DM 大鼠模型，收集粪便样本并提取 DNA，构建 16S rRNA 微生物基因克隆文库，进行高通量测序和生物信息学分析。结果表明，T1DM 大鼠与感染和炎症相关的致病细菌（如 Ruminococcaceae、Shigella、Enterococcus、Streptococcus、Rothia 和 Alistipes）丰度上调，而有益细菌（如 Lactobacillus、Faecalitalea、Butyricicoccus 和 Allobaculum）丰度降低。其中，Butyricicoccus 和 Allobaculum 通过产生短链脂肪酸来保护肠道屏障功能。本研究提示，肠道微生物群失衡导致的肠道炎症和短链脂肪酸（SCFAs）减少对 T1DM 的发病机制至关重要。
