Ansiliero R, Gelinski J M L N, Samistraro Q L, Baratto C M, Almeida C A, Locatelli C
Laboratory of Biotechnology, Universidade do Oeste de Santa Catarina, Videira, SC 89560000 Brazil.
Health Sciences, Uniarp, Caçador, Santa Catarina Brazil.
Indian J Microbiol. 2021 Mar;61(1):55-65. doi: 10.1007/s12088-020-00914-2. Epub 2021 Jan 1.
Phenotyping based on conventional microbiological, physiological, and molecular analysis by using ARDRA technique was developed with the aim to assess the pathogenic microbial load associated with different stages of the periodontal disease. In addition, in the face of the global issue of antimicrobial resistance, the isolated bacterial strains were evaluated for their antibiotic susceptibility profile. The pathogenic bacterial community was predominantly of Gram-negative strains (66.66%). The most common species were sp., sp., and sp. However, except for the healthy control group, spp. was isolated from all stages of periodontitis. Multidrug resistance to beta-lactam antibiotics was observed for sp. and . Here, we verify a statistically significant relationship between periodontitis stages and the diversity of the bacterial community. Patients with periodontitis showed a more diverse and numerous bacterial community compared to healthy patients. In this sense, we reinforce that biofilms that harbour multidrug-resistant bacteria are a major concern in relation to restoring patient health. Thus, prophylactic measures for maintaining oral health are still the best option for reduce the risk of disease.
基于传统微生物学、生理学以及使用ARDRA技术进行分子分析的表型分析方法得以开发，目的是评估与牙周疾病不同阶段相关的致病微生物负荷。此外，面对全球抗生素耐药性问题，对分离出的细菌菌株进行了抗生素敏感性分析。致病细菌群落主要为革兰氏阴性菌（66.66%）。最常见的菌种为[具体菌种1]、[具体菌种2]和[具体菌种3]。然而，除健康对照组外，[特定菌种]在牙周炎的各个阶段均有分离。观察到[具体菌种1]和[具体菌种2]对β-内酰胺类抗生素具有多重耐药性。在此，我们证实了牙周炎阶段与细菌群落多样性之间存在统计学上的显著关系。与健康患者相比，牙周炎患者的细菌群落更加多样且数量更多。从这个意义上讲，我们强调含有多重耐药菌的生物膜是恢复患者健康的主要关注点。因此，保持口腔健康的预防措施仍然是降低疾病风险的最佳选择。
