Laboratory of Pharmacognosy and Phytochemistry-Federal University of Amapá-10 Highway Jucelino Kubistichek, Km-02. Garden Zero-CEP: 68.902-280, Macapá, AP, Brazil.
Laboratory of Entomology Madical do Institute de Sciense Scientists and Technicians of the State of Amapá (IEPA), Highway Jucelino Kubistichek, Km-10. Fazendinha-CEP: 68.903-419 /68.903-197, Macapá, AP, Brazil.
ScientificWorldJournal. 2021 Jan 12;2021:2172919. doi: 10.1155/2021/2172919. eCollection 2021.
The present study evaluated the antioxidant, cytotoxic, and larvicidal potential of the essential oil of leaves, as well as identified the compounds present in the essential oil. The antioxidant activity was evaluated by the sequestration method of 2,2-diphenyl-1-picrylhydrazyl radical, the cytotoxic activity was evaluated using , the larvicidal bioassay was performed with larvae in the third stage of development of the mosquito, and the identification of the metabolites was performed by gas chromatography coupled to the mass spectrometer (GC-MS). The phytochemical oil analysis showed the presence of 20 compounds, with thymol and -terpinene being the main ones. It presented antioxidant activity with an IC of 194.51 g mL, demonstrating antioxidant activity in the highest concentrations tested. It presented low cytotoxic activity against , with an LC of 1238.67 g mL, demonstrating atoxicity in the concentrations tested. The essential oil presented good larvicidal activity when compared to the literature, with an LC = 79.0 g mL in 24 hours and LC of 69.15 g mL in 48 hours. In this way, it was possible to identify that the essential oil of the leaves of presented potential for the development of a natural larvicide, as well as antioxidant activity satisfactory to the radical DPPH and low toxicity to .
本研究评估了 的叶子精油的抗氧化、细胞毒性和杀幼虫潜力，并鉴定了精油中的化合物。抗氧化活性通过 2,2-二苯基-1-苦基肼自由基的螯合方法进行评估，细胞毒性活性使用 进行评估，幼虫在 的第三阶段发育阶段进行杀幼虫生物测定，代谢产物的鉴定通过气相色谱-质谱联用仪 (GC-MS) 进行。植物化学油分析表明存在 20 种化合物，其中百里酚和 -松油烯是主要成分。它具有 194.51 g mL 的 IC，表现出最高浓度测试下的抗氧化活性。它对 表现出低细胞毒性活性，LC 为 1238.67 g mL，在测试浓度下表现出无毒性。与文献相比，精油表现出良好的杀幼虫活性，在 24 小时时 LC = 79.0 g mL，在 48 小时时 LC 为 69.15 g mL。通过这种方式，我们可以确定 的叶子精油具有开发天然杀虫剂的潜力，以及对 DPPH 自由基的抗氧化活性令人满意，对 毒性低。
