Lee Haesu, Kim Mi Hye, Jin Seong Chul, Han Jae Min, Park Jun Hyuk, Yang Woong Mo
Department of Convergence Korean Medical Science, College of Korean Medicine, Graduate School, Kyung Hee University, 26 Kyungheedae-ro, Dongdaemun-gu, Seoul 02447, Republic of Korea.
Evid Based Complement Alternat Med. 2021 Jan 6;2021:7347639. doi: 10.1155/2021/7347639. eCollection 2021.
Localized adiposity is not only a common aesthetic issue but also a health risk factor. Pharmacopuncture can be a therapeutic option for the imbalance of regional fat distribution. The tuber of has been prescribed as antitussive and expectorant as a traditional Korean medicine. This study investigated the effects of pharmacopuncture with water extract (PT) on localized adiposity. Male C57BL/6J mice were fed on a high-fat diet (HFD) for 6 weeks. 100 L of 10 mg/mL of PT was injected into the left-side inguinal fat pad, while saline was injected into the right-side inguinal fat pad as self-control. Treatments were performed 3 times per week for 4 weeks. The inguinal fat weight was analyzed by dual-energy X-ray absorptiometry. PT pharmacopuncture significantly decreased the weight of the inguinal fat pad. The adipocyte size was reduced with increases of lipolytic enzymes and lipophagy-related factors by PT pharmacopuncture. There was marked inhibition of lipid accumulation content in 3T3-L1 adipocytes by PT treatment. The expressions of adipose triglyceride lipase (ATGL), hormone-sensitive lipase (HSL), autophagy-related gene (ATG) 5, ATG7, and LC3 were markedly increased by PT treatments and . This study suggests that pharmacopuncture of has ameliorative effects on adiposity by lipid catabolic effects via activating both lipolysis and lipophagy in a localized region.
局部肥胖不仅是一个常见的美学问题，也是一个健康风险因素。药物穴位注射可以作为解决局部脂肪分布失衡的一种治疗选择。作为传统的韩国药物，[植物名称]的块茎已被用作止咳祛痰药。本研究调查了[植物名称]水提取物（PT）药物穴位注射对局部肥胖的影响。雄性C57BL/6J小鼠喂食高脂饮食（HFD）6周。将100μL 10mg/mL的PT注射到左侧腹股沟脂肪垫中，而将生理盐水注射到右侧腹股沟脂肪垫中作为自身对照。每周进行3次治疗，持续4周。通过双能X射线吸收法分析腹股沟脂肪重量。PT药物穴位注射显著降低了腹股沟脂肪垫的重量。PT药物穴位注射通过增加脂肪分解酶和脂肪自噬相关因子，使脂肪细胞大小减小。PT处理显著抑制了3T3-L1脂肪细胞中的脂质积累含量。PT处理[具体处理方式1]和[具体处理方式2]后，脂肪甘油三酯脂肪酶（ATGL）、激素敏感性脂肪酶（HSL）、自噬相关基因（ATG）5、ATG7和LC3的表达显著增加。本研究表明，[植物名称]的药物穴位注射通过激活局部区域的脂肪分解和脂肪自噬，对肥胖具有改善作用，通过脂质分解作用发挥功效。
