Lee Haesu, Kim Mi Hye, Jin Seong Chul, Choi La Yoon, Nam Yeon Kyung, Yang Woong Mo
Department of Convergence Korean Medical Science, College of Korean Medicine, Kyung Hee University, Seoul 02447, Republic of Korea.
Boin Bio Convergence Co., Ltd., Seoul 02455, Republic of Korea.
Exp Ther Med. 2021 Nov;22(5):1290. doi: 10.3892/etm.2021.10725. Epub 2021 Sep 13.
Localized adiposity is a serious aesthetic problem and a well-known health risk factor. There is a growing interest in minimally invasive treatment options for excessive fat accumulation, such as pharmacopuncture. LIPOSA is a newly developed pharmacopuncture formula from three natural herbs: The tuber of (Thunb.) Breitenb., the whole plant of Dahlst. and the root of Bunge. The present study investigated the effects of pharmacopuncture treatment with LIPOSA on localized adiposity. Male C57BL/6J mice were fed high fat diet for 8 weeks to induce obesity. Then, 100 µl LIPOSA was injected into the left-side inguinal fat pad at various concentrations, including 13.35, 26.7 and 53.4 mg/ml. Normal saline was injected into the right-side inguinal fat pad of each mouse as a control. The treatment was performed three times per week for 2 weeks. The weight and histological changes were analyzed in the inguinal fat pad of the obese mice. The expression levels of adipose triglyceride lipase (ATGL), hormone-sensitive lipase (HSL), autophagy-related gene (ATG)5, ATG7 and LC3-II, as lipophagy-related factors, were evaluated to confirm the lipid-catabolic effects of LIPOSA. LIPOSA pharmacopuncture markedly decreased the weight of the fat tissue and the size of the adipocytes in the inguinal region of the mouse models of obesity in a dose-dependent manner. The expression levels of ATGL, HSL, ATG5, ATG7 and LC3-II were significantly increased by the LIPOSA treatments. In addition, LIPOSA pharmacopuncture was found to decrease the expression levels of ACC, PPAR-γ and PEPCK. The results indicated that subcutaneous injection of LIPOSA can degrade local fat and induce lipophagic and lipase activation effects. In addition, lipid metabolism related to fat accumulation was regulated by the LIPOSA treatment. The present study suggests that LIPOSA pharmacopuncture can be a non-surgical alternative in the treatment of localized adiposity.
局部肥胖是一个严重的美学问题，也是一个众所周知的健康风险因素。人们对针对过多脂肪堆积的微创治疗方法，如药物穴位注射，的兴趣与日俱增。LIPOSA是一种新开发的由三种天然草药制成的药物穴位注射配方：（Thunb.）Breitenb.的块茎、Dahlst.的全株以及Bunge的根。本研究调查了用LIPOSA进行药物穴位注射治疗对局部肥胖的影响。雄性C57BL/6J小鼠喂食高脂肪饮食8周以诱导肥胖。然后，将100微升不同浓度（包括13.35、26.7和53.4毫克/毫升）的LIPOSA注射到左侧腹股沟脂肪垫中。将生理盐水注射到每只小鼠的右侧腹股沟脂肪垫中作为对照。每周进行三次治疗，持续2周。对肥胖小鼠的腹股沟脂肪垫进行重量和组织学变化分析。评估脂肪甘油三酯脂肪酶（ATGL）、激素敏感性脂肪酶（HSL）、自噬相关基因（ATG）5、ATG7和LC3-II作为脂肪自噬相关因子的表达水平，以确认LIPOSA的脂质分解作用。LIPOSA药物穴位注射以剂量依赖的方式显著降低了肥胖小鼠模型腹股沟区域脂肪组织的重量和脂肪细胞的大小。LIPOSA治疗显著提高了ATGL、HSL、ATG5、ATG7和LC3-II的表达水平。此外，发现LIPOSA药物穴位注射可降低ACC、PPAR-γ和PEPCK的表达水平。结果表明，皮下注射LIPOSA可降解局部脂肪并诱导脂肪自噬和脂肪酶激活作用。此外，LIPOSA治疗调节了与脂肪堆积相关的脂质代谢。本研究表明，LIPOSA药物穴位注射可成为治疗局部肥胖的非手术替代方法。
