糖皮质激素可抑制脂多糖刺激的人单核细胞产生肿瘤坏死因子。
Glucocorticoids suppress the production of tumour necrosis factor by lipopolysaccharide-stimulated human monocytes.
作者信息
Waage A, Bakke O
机构信息
Cell Research Laboratory, University of Trondheim, Norway.
出版信息
Immunology. 1988 Feb;63(2):299-302.
We have investigated the modulating effect of steroids on the in vitro production of tumour necrosis factor (TNF) by lipopolysaccharide (LPS)-stimulated human monocytes. Dexamethasone, at concentrations ranging from 10(-8) to 10(-6) M, and cortisol, at concentrations 10(-7) and 10(-6) M, suppressed the TNF production in a dose-dependent manner. The highest concentrations of dexamethasone or cortisol reduced the TNF production to 21 +/- 2% and 48 +/- 8% of the control value, respectively. The effect of dexamethasone was time dependent, and an incubation time of 48 hr was required to reduce the TNF production to 21% of control. The effect of dexamethasone decreased when the incubation time became shorter, and the mean TNF production ranged from 49% to 72% of control when dexamethasone was added later than 8 hr before LPS addition, at the time of LPS addition, or within 1 hr after LPS addition. The magnitude of the TNF-suppressing effect of dexamethasone varied greatly from donor to donor. Only the glucocorticoids, and not the sex steroids or the mineralocorticoids, significantly reduced the TNF production.
我们研究了类固醇对脂多糖（LPS）刺激的人单核细胞体外产生肿瘤坏死因子（TNF）的调节作用。地塞米松浓度范围为10^(-8)至10^(-6)M，皮质醇浓度为10^(-7)和10^(-6)M，均以剂量依赖方式抑制TNF产生。地塞米松或皮质醇的最高浓度分别将TNF产生量降至对照值的21±2%和48±8%。地塞米松的作用具有时间依赖性，需要48小时的孵育时间才能将TNF产生量降至对照的21%。当孵育时间缩短时，地塞米松的作用减弱，当地塞米松在LPS添加前8小时后、LPS添加时或LPS添加后1小时内添加时，TNF的平均产生量为对照的49%至72%。地塞米松对TNF的抑制作用程度在不同供体之间差异很大。只有糖皮质激素能显著降低TNF产生，而性激素或盐皮质激素则不能。
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