对精液中负责抑制自然杀伤细胞活性的因素的鉴定。
The identification of factors in seminal plasma responsible for suppression of natural killer cell activity.
作者信息
Vallely P J, Sharrard R M, Rees R C
机构信息
Department of Virology, University of Sheffield Medical School, U.K.
出版信息
Immunology. 1988 Mar;63(3):451-6.
We have identified two components of human seminal plasma which suppress natural killer (NK) cell activity in vitro. Dialysis and gel filtration experiments have shown both components to be of low molecular weight. The first will suppress NK cells following a short period of pretreatment, but this suppression is dependent upon the presence of bovine serum in the medium and is directly related to a loss of cell viability as measured by trypan blue dye exclusion. We suggest that this molecule is a polyamine. The second factor will not suppress NK activity following pretreatment of lymphocytes, but is a potent suppressor when added for the duration of the assay. This suppression is completely bovine serum independent, unrelated to toxicity and appears to be mediated by prostaglandin E2. The relevance of these results to a clinical situation is discussed.
我们已经鉴定出人类精浆中的两种成分，它们在体外可抑制自然杀伤（NK）细胞的活性。透析和凝胶过滤实验表明这两种成分的分子量都很低。第一种成分在短时间预处理后会抑制NK细胞，但这种抑制依赖于培养基中牛血清的存在，并且与用台盼蓝染料排除法测定的细胞活力丧失直接相关。我们认为这种分子是一种多胺。第二种因子在淋巴细胞预处理后不会抑制NK活性，但在检测期间添加时是一种有效的抑制剂。这种抑制完全不依赖牛血清，与毒性无关，并且似乎是由前列腺素E2介导的。本文讨论了这些结果与临床情况的相关性。
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