Bischof P, Martin-Du-Pan R, Lauber K, Girard J P, Herrmann W L, Sizonenko P C
J Clin Endocrinol Metab. 1983 Feb;56(2):359-62. doi: 10.1210/jcem-56-2-359.
Pregnancy-associated plasma protein-A (PAPP-A) was detected by RIA in human seminal plasma. This was not due to interference with proteases or binding to seminal plasma proteins, since immunoreactivity was not affected by treatment with protease inhibitors, and the elution of [125I]PAPP-A was not altered by preincubation with seminal plasma. The major component of the seminal plasma PAPP-A coeluted with pure PAPP-A or plasma PAPP-A from pregnant and nonpregnant women. In the RIA, serial dilutions of seminal plasma gave parallel displacement curves to pregnancy plasma. Removing PAPP-A-like material from seminal plasma by adsorbtion to heparin-Sepharose reduced the inhibitory effect of seminal plasma on phytohemagglutinin-induced lymphocyte transformation. The source of seminal plasma PAPP-A-like immunoreactive material remains to be elucidated, but it is unlikely to be the testis, since PAPP-A levels in semen of vasectomized men were similar to those in nonvasectomized men.
采用放射免疫分析法（RIA）检测人精浆中的妊娠相关血浆蛋白-A（PAPP-A）。这并非由于蛋白酶干扰或与精浆蛋白结合所致，因为免疫反应性不受蛋白酶抑制剂处理的影响，并且[125I]PAPP-A的洗脱也不会因与精浆预孵育而改变。精浆PAPP-A的主要成分与来自孕妇和非孕妇的纯PAPP-A或血浆PAPP-A共洗脱。在放射免疫分析中，精浆的系列稀释产生了与妊娠血浆平行的置换曲线。通过与肝素-琼脂糖吸附去除精浆中类似PAPP-A的物质，可降低精浆对植物血凝素诱导的淋巴细胞转化的抑制作用。精浆中类似PAPP-A免疫反应性物质的来源尚待阐明，但不太可能是睾丸，因为输精管结扎男性精液中的PAPP-A水平与未结扎男性相似。
