Arbizu Ricardo A, Collins David, Wilson Robert C, Alekseyenko Alexander V
Division of Pediatric Gastroenterology, Department of Pediatrics, Medical University of South Carolina, Charleston, SC, USA.
South Carolina Clinical and Translational Research Institute, Charleston, SC, USA.
Pediatr Gastroenterol Hepatol Nutr. 2021 Jan;24(1):30-37. doi: 10.5223/pghn.2021.24.1.30. Epub 2021 Jan 8.
To investigate the differences in the colon microbiota composition of Hirschsprung's disease (HSCR) patients with and without a history of postoperative Hirschsprung's associated enterocolitis (HAEC).
Colon tissue microbiota was characterized by bacterial deoxyribonucleic acid (DNA) extraction and 16S rDNA sequencing for taxonomic classification and comparison.
The sequence diversity richness within samples was significantly higher in samples from patients with a history of postoperative HAEC. We observed an increased relative abundance of the phyla , and in HAEC patients and , and in HSCR patients and, an increased relative abundance of the genera , and in HAEC patients and and in HSCR patients.
Our findings provide evidence that the colon tissue microbiota composition is different in HSCR patients with and without postoperative HAEC.
研究有和没有术后先天性巨结肠相关小肠结肠炎（HAEC）病史的先天性巨结肠（HSCR）患者结肠微生物群组成的差异。
通过细菌脱氧核糖核酸（DNA）提取和16S核糖体DNA测序对结肠组织微生物群进行特征分析，以进行分类和比较。
有术后HAEC病史患者的样本中，样本内的序列多样性丰富度显著更高。我们观察到，HAEC患者中门 、 和 的相对丰度增加，HSCR患者中门 、 和 的相对丰度增加；HAEC患者中属 、 和 的相对丰度增加，HSCR患者中属 和 的相对丰度增加。
我们的研究结果证明，有和没有术后HAEC的HSCR患者的结肠组织微生物群组成不同。
