Arnaud Alexis P, Cousin Ianis, Schmitt Françoise, Petit Thierry, Parmentier Benoit, Levard Guillaume, Podevin Guillaume, Guinot Audrey, DeNapoli Stéphan, Hervieux Erik, Flaum Valérie, De Vries Philine, Randuineau Gwénaëlle, David-Le Gall Sandrine, Buffet-Bataillon Sylvie, Boudry Gaëlle
Institut NuMeCan, INRAE, INSERM, Univ Rennes, Rennes-Saint-Gilles, France.
Department of Pediatric Surgery, CHU Rennes, Univ Rennes, Rennes, France.
Front Microbiol. 2022 Jun 30;13:904758. doi: 10.3389/fmicb.2022.904758. eCollection 2022.
Patients with Hirschsprung's disease are at risk of developing Hirschsprung-associated enterocolitis, especially in the first 2 years of life. The pathophysiology of this inflammatory disease remains unclear, and intestinal dysbiosis has been proposed in the last decade. The primary objective of this study was to evaluate in a large cohort if Hirschsprung-associated enterocolitis was associated with alterations of fecal bacterial composition compared with HD without enterocolitis in different age groups.
We analyzed the fecal microbiota structure of 103 Hirschsprung patients from 3 months to 16 years of age, all of whom had completed definitive surgery for rectosigmoid Hirschsprung. 16S rRNA gene sequencing allowed us to compare the microbiota composition between Hirschsprung's disease patients with (HAEC group) or without enterocolitis (HD group) in different age groups (0-2, 2-6, 6-12, and 12-16 years).
Richness and diversity increased with age group but did not differ between HD and HAEC patients, irrespective of the age group. Relative abundance of Actinobacteria was lower in HAEC than in HD patients under 2 years of age (-66%, = 0.045). Multivariate analysis by linear models (MaAsLin) considering sex, medications, birth mode, breast-feeding, and the Bristol stool scale, as well as surgery parameters, highlighted and , as well as group, as positively associated with Hirschsprung-associated enterocolitis in the 0-2 years age group.
Hirschsprung-associated enterocolitis was associated with features of intestinal dysbiosis in infants (0-2 years) but not in older patients. This could explain the highest rate of enterocolitis in this age group.
https://clinicaltrials.gov/ct2/show/NCT02857205, MICROPRUNG, NCT02857205, 02/08/2016.
先天性巨结肠症患者有发生先天性巨结肠相关小肠结肠炎的风险，尤其是在生命的头两年。这种炎症性疾病的病理生理学仍不清楚，在过去十年中有人提出肠道微生物群失调与之有关。本研究的主要目的是在一个大型队列中评估，与不同年龄组未患小肠结肠炎的先天性巨结肠症患者相比，先天性巨结肠相关小肠结肠炎是否与粪便细菌组成的改变有关。
我们分析了103例年龄在3个月至16岁之间的先天性巨结肠症患者的粪便微生物群结构，所有患者均已完成乙状结肠直肠先天性巨结肠症的确定性手术。16S rRNA基因测序使我们能够比较不同年龄组（0 - 2岁、2 - 6岁、6 - 12岁和12 - 16岁）患有（先天性巨结肠相关小肠结肠炎组）或未患小肠结肠炎（先天性巨结肠症组）的先天性巨结肠症患者之间的微生物群组成。
丰富度和多样性随年龄组增加，但无论年龄组如何，先天性巨结肠症患者和先天性巨结肠相关小肠结肠炎患者之间均无差异。2岁以下先天性巨结肠相关小肠结肠炎患者中放线菌的相对丰度低于先天性巨结肠症患者（-66%，P = 0.045）。通过线性模型（MaAsLin）进行多变量分析，考虑性别、药物、出生方式、母乳喂养、布里斯托大便分类法以及手术参数，突出显示在0 - 2岁年龄组中，[具体细菌名称1]、[具体细菌名称2]以及[具体细菌组]与先天性巨结肠相关小肠结肠炎呈正相关。
先天性巨结肠相关小肠结肠炎与婴儿（0 - 2岁）肠道微生物群失调特征有关，但与年龄较大患者无关。这可以解释该年龄组小肠结肠炎发生率最高的原因。
https://clinicaltrials.gov/ct2/show/NCT02857205，MICROPRUNG，NCT02857205，2016年8月2日。
