Jorgensen Michael D, Chmielewski Jean
Department of Chemistry, Purdue University, West Lafayette, Indiana, USA.
J Pept Sci. 2022 Jan;28(1):e3302. doi: 10.1002/psc.3302. Epub 2021 Jan 27.
Mimicking the extracellular matrix (ECM) continues to be a goal in the field of regenerative medicine. Herein, we report a modified trimeric GCN4 coiled-coil sequence containing three ligands for metal ions specifically positioned for crosslinked assembly (TriCross). In the presence of metal ions, TriCross assembles into a three-dimensional (3D) matrix with significant cavities to accommodate cells. The matrix was found to be stable in media with serum, and mild removal of the metal leads to disassembly. By assembling TriCross with a suspension of cells in media, the matrix encapsulates cells during the assembly process leading to high cell viability. Further disassembly under mild conditions allows for the release of cells from the scaffold. As such, this peptide-based material displays many of the characteristics necessary for successful 3D cell culture.
模仿细胞外基质（ECM）仍然是再生医学领域的一个目标。在此，我们报告了一种修饰的三聚体GCN4卷曲螺旋序列，其包含三个特定定位用于交联组装的金属离子配体（TriCross）。在金属离子存在的情况下，TriCross组装成具有大量空隙以容纳细胞的三维（3D）基质。发现该基质在含血清的培养基中稳定，并且温和去除金属会导致其解体。通过将TriCross与培养基中的细胞悬液组装，基质在组装过程中包裹细胞，从而导致高细胞活力。在温和条件下进一步解体允许细胞从支架中释放。因此，这种基于肽的材料展现出成功进行3D细胞培养所需的许多特性。
