Nambiar Monessha, Nepal Manish, Chmielewski Jean
Department of Chemistry, Purdue University, 560 Oval Drive, West Lafayette, Indiana 47907, United States.
ACS Biomater Sci Eng. 2019 Oct 14;5(10):5082-5087. doi: 10.1021/acsbiomaterials.9b01304. Epub 2019 Sep 30.
We report a coiled-coil trimeric peptide based on the known GCN4 zipper motif that self-assembles into nanotubes with diameters on the order of a few hundred nanometers to a micron. The dimensional morphology of these tubular structures was observed to be tunable by altering the properties of the buffer during assembly. Structural evidence from X-ray scattering and electron microscopy suggest that tube assembly takes place in structural tiers leading to a hexagonal close-packed arrangement of the coiled-coils. The hollow tubes were observed to selectively encapsulate fluorescein-labeled anionic dextran, as a result of electrostatic interactions between the inner tube surface and negatively charged cargo. The ability of these nanotubes to house biomolecular cargo endows them with potential for molecular storage and drug delivery applications.
我们报道了一种基于已知GCN4拉链基序的卷曲螺旋三聚体肽，它能自组装成直径在几百纳米到一微米量级的纳米管。通过改变组装过程中缓冲液的性质，可以观察到这些管状结构的尺寸形态是可调的。X射线散射和电子显微镜的结构证据表明，管的组装是在结构层中进行的，导致卷曲螺旋呈六方密堆积排列。由于管内表面与带负电荷的货物之间的静电相互作用，观察到空心管能选择性地包裹荧光素标记的阴离子葡聚糖。这些纳米管容纳生物分子货物的能力赋予了它们在分子存储和药物递送应用方面的潜力。
