Department of Urology, Beilun District People's Hospital, Ningbo, China.
Eur Rev Med Pharmacol Sci. 2021 Jan;25(1):108-115. doi: 10.26355/eurrev_202101_24354.
Long non-coding RNA (lncRNA) was frequently abnormally expressed in cancers. LINC00641 was reported to play crucial roles in regulating tumor progression. However, its role in prostate cancer (PCa) has not been fully explored.
In this work, proliferation, invasion and apoptosis assays were performed to detect the biological roles of LINC00641 in PCa. Bioinformatic analyses, Luciferase activity reporter assay, and rescue experiments were performed to investigate the potential mechanisms of LINC00641 in PCa. Expression levels of LINC00641, microRNA-365a-3p (miR-365a-3p), and vestigial like family member 4 (VGLL4) in PCa tissues and normal tissues were analyzed at ENCORI.
We found LINC00641 and VGLL4 was reduced, while miR-365a-3p was elevated expression in PCa tissues compared with normal tissues. LINC00641 overexpression inhibited growth and invasion abilities of PCa cells in vitro. Functional assays revealed that miR-365a-3p/VGLL4 pair was the downstream targets of LINC00641.
The findings of our work provided evidence that LINC00641 serves as a tumor suppressive lncRNA in PCa by regulating miR-365a-3p/VGLL4 axis.
长链非编码 RNA（lncRNA）在癌症中常异常表达。LINC00641 被报道在调节肿瘤进展中发挥关键作用。然而，其在前列腺癌（PCa）中的作用尚未被充分探索。
在这项工作中，进行了增殖、侵袭和凋亡实验，以检测 LINC00641 在 PCa 中的生物学作用。进行了生物信息学分析、荧光素酶活性报告实验和挽救实验，以研究 LINC00641 在 PCa 中的潜在机制。在 ENCORI 分析了 PCa 组织和正常组织中 LINC00641、微小 RNA-365a-3p（miR-365a-3p）和 vestigial 样家族成员 4（VGLL4）的表达水平。
我们发现与正常组织相比，PCa 组织中 LINC00641 和 VGLL4 的表达降低，而 miR-365a-3p 的表达升高。LINC00641 的过表达抑制了 PCa 细胞在体外的生长和侵袭能力。功能实验表明，miR-365a-3p/VGLL4 对是 LINC00641 的下游靶标。
我们的研究结果提供了证据，表明 LINC00641 通过调节 miR-365a-3p/VGLL4 轴在 PCa 中作为一种肿瘤抑制性 lncRNA 发挥作用。
